双参扶正散效应物质分析及其对人肺腺癌A549细胞的机制研究OA

Non-targeted TCM metabolomic techniques were employed to explore the potential effective substances of Shuangshen Fuzheng San.The inhibitory effects of Shuangshen Fuzheng San and its effective substances on human lung adenocarcinoma A549 cells in vitro were evaluated by using CCK-8 assays,colony formation assays,flow cytometric analysis of apoptosis,and Western blot analysis of apoptosis-related proteins.This study aims to explore the optimal effective substance combination,further analyze,and verify the potential action mechanisms underlying the inhibitory effects of Shuangshen Fuzheng San and its effective substances on the lung adenocarcinoma through network pharmacology analysis.The results demonstrate that ginsenoside Rg3,ginsenoside Ro,and notoginsenoside Fe can simultaneously enter into the mice's serum and lung tissues and are identified as the key effective substances of Shuangshen Fuzheng San responsible for inhibiting A549 cell proliferation.The combination of ginsenoside Rg3 and notoginsenoside Fe exhibits the strongest inhibitory effect on A549 cell proliferation and is determined to be the optimal effective substance combination.The optimal effective substance combination can inhibit clonogenic formation of A549 cells,significantly promote the apoptosis of A549 cells,and markedly upregulate the expression of apoptosis-related proteins,with the effective substance combination showing a superior effect compared with the drug-containing serum.The interleukin(IL)-17 signaling pathway is strongly associated with the inhibitory effects of Shuangshen Fuzheng San on lung adenocarcinoma.Consistently,both the optimal effective substance combination of Shuangshen Fuzheng San and drug-containing serum can significantly downregulate the expression levels of key IL-17 pathway-related proteins,including IL-17A,IL-6,and matrix metalloproteinase-9(MMP-9),in A549 cells.Collectively,ginsenoside Rg3,ginsenoside Ro,and notoginsenoside Fe are the key effective substances of Shuangshen Fuzheng San.The combination of ginsenoside Rg3 and notoginsenoside Fe constitutes the optimal effective substance combination of Shuangshen Fuzheng San,which together with drug-containing serum,inhibits lung adenocarcinoma progression via the IL-17 signaling pathway and exhibits superior inhibitory effects on lung adenocarcinoma in vitro compared with drug-containing serum.