两个香菇品种贮藏特性及蛋白组学的比较分析OA

To explore the biological mechanisms underlying the storability of Lentinula edodes and screen key proteins as-sociated with storability,this study employed the storage-tolerant variety Yuxiang 1(YX)and the storage-intolerant vari-ety 9608(JL)as test materials.A systematic comparison was conducted on their physiological indicators including storage characteristics,cell membrane stability,antioxidant capacity,cell wall components,and sugar metabolism,combined with proteomic techniques for differential analysis.The results showed that during storage,YX consistently maintained low wa-ter loss rate,browning degree,and respiratory rate,with a slow decrease in firmness.Moreover,its relative electrical con-ductivity and malondialdehyde(MDA)content were significantly lower than those of JL,while the activity of superoxide dismutase(SOD)and catalase(CAT)remained at a high level.Regarding cell wall components,YX had higher content of cellulose and chitin which degraded slowly,whereas the activity of cellulase and chitinase were significantly lower than those of JL.In terms of sugar metabolism,the total soluble sugar content of YX decreased less significantly,and the con-tent of key components such as trehalose were significantly higher than those of JL.Proteomic analysis identified a total of 5474 proteins,and the number of differentially expressed proteins(DEPs)in YX before and after storage(95)was much fewer than that in JL(789),indicating stronger stability of protein expression in the storage-tolerant variety.Further screening revealed that glucanase(LENED_012880)and agmatinase(LENED_003685)were key storability-related pro-teins,both of which were significantly up-regulated during the storage of YX.GO and KEGG enrichment analysis demon-strated that these key proteins were mainly involved in pathways such as cell wall organization,glycerophospholipid me-tabolism,and starch and sucrose metabolism.In conclusion,YX achieves excellent storability through the synergistic ef-fects of maintaining a low metabolic level,enhancing the antioxidant defense system,and stabilizing cell wall structure.The key storability-related proteins screened in this study provide core targets for the molecular breeding of storage-toler-ant L.edodes varieties,and the relevant results also offer theoretical support for deciphering the regulatory mechanisms of postharvest senescence in L.edodesand optimizing preservation technologies.