不同消毒方式对鸽种蛋灭菌及孵化效果的影响OA

[Objective]In order to explore the effects of different disinfection methods on the microorganisms eradication on the eggshell surface and hatching effect of pigeon eggs.[Method]This experiment established five disinfection methods.Using the same incubator and hatching program,15000 pigeon hatching eggs were selected and randomly divided into five groups,with 3 replicates per group and 1,000 eggs per replicate.Each group was disinfected with the corresponding method before incubation.The specific groups were designed as follows:formaldehyde+potassium permanganate fumigation(Group A),bromogeramine solution immersion(Group B),bromogeramine solution spraying(Group C),potassium permanganate solution immersion(Group D),and trichloroisocyanuric acid fumigation(Group E).The surface Salmonella,Escherichia coli(E.coli),and total bacterial counts on the eggshells before and after disinfection were measured,and the sterilization rates were calculated.Hatching-related parameters including infertile eggs,dead embryos,healthy squabs,weak squabs and dead squabs were recorded.[Result]The results showed that Salmonella was not detected in the experiment,and no E.coli colonies were found in all groups after disinfection.For total bacterial sterilization rate,Group B and C were significantly greater than the other groups(P<0.05),and Group A and E were significantly greater than Group D(P<0.05).In terms of hatching performance,Group C had the significantly highest dead embryo rate and the significantly lowest hatching rate of fertile eggs(P<0.05),while Group E had the significantly lowest dead embryo rate(P<0.05).The hatching rates of fertile eggs in Group A and E were significantly greater than those other groups C and D(P<0.05).For chick performance,Group C had the significantly lowest healthy chick rate and the significantly highest dead chick rate(P<0.05),whereas Group A and E had the significantly highest healthy chick rate(P<0.05).The dead chick rate of Group B was significantly greater than that of Group A,D and E(P<0.05).[Conclusion]In conclusion,Group B and C showed better sterilization effects,while Group A and E exhibited better hatching and chick performance.Group C presented the poorest overall effect.Group A achieved a balance between sterilization efficiency and hatching performance,representing the optimal comprehensive treatment.