绵羊CYP17A1基因CDS区分子特征分析及其在不同时期睾丸中的表达研究OA

[Objective]This study was conducted to investigate the dynamic expression characteristics of CYP17A1 during testicular development in Small-tail han sheep,clarify its biological function and expression pattern,and provide a theoretical reference for further research.[Method]In this study,multiple bioinformatics tools were used to analyze the molecular characteristics of sheep CYP17A1 and construct a phylogenetic tree.Meanwhile,quantitative real-time polymerase chain reaction(qRT-PCR)and Western blot were performed to detect the expression of CYP17A1 in the testes of Small-Tail Han sheep at 2,6,and 12 months of age.[Result]The full length of the CDS region of the sheep CYP17A1 gene is 1576 bp,and it shows the closest genetic relationship with ruminants such as goats and cattle.The gene encodes 509 amino acids.The molecular formula of the CYP17A1 protein is C 2605 H 4140 N 696 S 15,with a molecular weight of 57.39 kDa and a theoretical isoelectric point of 8.60.It is a slightly hydrophilic protein that contains phosphorylation sites but has no signal peptide.The secondary and tertiary structures of CYP17A1 are mainly composed of α-helix,β-sheet,extended strand,and random coil.Both the CYP17A1 gene and its protein were expressed in testicular tissue at all three developmental stages,and their expression levels showed a gradually decreasing trend with increasing age:the highest expression was observed at 2 months of age,followed by 6 months of age,and the lowest at 12 months of age.[Conclusion]Taken together,these findings demonstrate that CYP17A1 is involved in and exerts an essential regulatory effect on testicular development in Small-tail han sheep.