基于色谱-质谱联用技术的咽立爽口含滴丸在大鼠胆汁、粪便、尿液中的代谢、排泄研究OA

To delineate the in vivo metabolism and excretion of Yanlishuang Oral Dripping Pills in rats,an integrated analytical strategy was implemented through chromatography-mass spectrometry technology.Biological samples(bile,feces,and urine)were collected before and after oral administration of Yanlishuang Oral Dripping Pills.Processed samples were analyzed by gas chromatography-mass spectrometry(GC-MS)and ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Through systematic comparison of retention time,mass-to-charge ratios,and characteristic fragment ions,a total of 4 prototype compounds and 30 metabolites were identified across biological matrices,including 18 metabolites in bile,13 in feces,and 24 in urine.Primary metabolic pathways involved hydrolysis,hydrogenation,methylation,sulfation,and glucuronidation.Quantitative analysis further determined cumulative excretion rates of prototype components(borneol,menthol,camphor,and glycyrrhizic acid)and their metabolites(borneol-2-O-glucuronide,menthol-1-O-glucuronide,glycyrrhetinic acid,and glycyrrhetinic acid-3-O-β-D-glucuronide)in bile,feces,and urine.This study systematically clarified the in vivo metabolism and excretion of Yanlishuang Oral Dripping Pills,providing critical experimental evidence for further clinical applications.