补阳还五汤及其不同部位调节胆固醇治疗高脂血症的作用机制研究OA
Mechanism of Buyang Huanwu Decoction and its different fractions in regulating cholesterol and treating hyperlipidemia
旨在探讨补阳还五汤及其不同部位治疗高脂血症的作用机制,筛选调血脂活性部位并优化配方.鉴定含药血清中的代谢产物,并进行高脂血症疾病富集分析.将 96 只 ICR 小鼠随机分成空白组 12 只、造模组 84 只,造模组小鼠高脂饲料喂养 6 周建立高脂血症模型后,随机分为模型组、阿托伐他汀组(2.600 mg·kg-1)、补阳还五汤组(18.571 g·kg-1)、醇沉组(1.496 g·kg-1)、30%乙醇组(0.163 g·kg-1)、50%乙醇组(0.113 g·kg-1)、90%乙醇组(0.060 g·kg-1),给药3 周后取材.检测小鼠血脂指标和肝脏胆固醇、甘油三酯、总胆汁酸水平,同时检测肝脏和脂肪病理变化及肝脏脂质相关基因[固醇调节元件结合蛋白 2(SREBP2)、胰岛素诱导基因-1(INSIG-1)、3-羟基-3-甲基戊二酰辅酶 A 还原酶(HMGCR)、肝 X 受体 α(LXRα)、ATP 结合盒转运体 A1(ABCA1)、胆固醇 7α-羟化酶(CYP7A1)]的 mRNA 与蛋白表达.结果表明,醇沉组与 30%乙醇组在降低胆固醇方面效果显著;醇沉组与 50%乙醇组对白色脂肪组织的调控作用更优.除 90%乙醇组外,其余各组肝脏胆固醇与甘油三酯水平均降低;所有给药均对总胆汁酸产生影响.补阳还五汤及其不同部位可调控肝脏脂质相关基因与蛋白的表达.综上,补阳还五汤、醇沉部位、30%乙醇部位、50%乙醇部位通过降低肝脏胆固醇有效治疗高脂血症,但作用机制存在差异:50%乙醇部位主要抑制胆固醇合成,醇沉与 30%乙醇部位侧重促进胆固醇外排,醇沉部位在胆固醇向胆汁酸转化方面优势显著.
This study aimed to explore the mechanisms of Buyang Huanwu Decoction(BYHWD)and its different fractions in treating hyperlipidemia,screen lipid-regulating active components,and optimize the formula ratio.Metabolites in drug-containing sera were identified.A total of 96 ICR mice were randomly divided into a blank group(12 mice)and a modeling group(84 mice).The modeling group was fed a high-fat diet for 6 weeks to establish a hyperlipidemia model,and then randomly divided into a model group,an atorvastatin group(2.600 mg·kg-1),a BYHWD group(18.571 g·kg-1),an alcohol precipitation group(1.496 g·kg-1),a 30%fraction group(0.163 g·kg-1),a 50%fraction group(0.113 g·kg-1),and a 90%fraction group(0.060 g·kg-1).Samples were collected after administration for three weeks.Serum lipid indices,hepatic cholesterol(CHO),triglyceride(TG),and total bile acid(TBA)levels were detected.Pathological changes in the liver and adipose tissue,as well as the mRNA and protein expression of lipid-related genes(sterol regulatory element-binding protein 2[SREBP2],insulin-induced gene 1 protein[INSIG-1],3-hydroxy-3-methylglutaryl-CoA reductase[HMGCR],liver X receptor alpha[LXRα],ATP-binding cassette transporter A1[ABCA1],and cytochrome P450 family 7 subfamily A member 1[CYP7A1])in the liver,were also determined.The results showed that the alcohol precipitation and 30%fraction showed significant effects on lowering CHO,and the alcohol precipitation and 50%fraction exhibited better regulatory effects on white adipose tissue.Except for the 90%fraction,all the other had reduced hepatic CHO and TG levels.All groups showed an impact on TBA.BYHWD and its different fractions could regulate the expression of lipid-related genes and proteins in the liver.In conclusion,the BYHWD,alcohol precipitation,30%fraction,and 50%fraction effectively treat hyperlipidemia by reducing hepatic CHO,but their mechanisms of action are different:the 50%fraction mainly inhibits CHO synthesis;the alcohol precipitation and 30%fraction focus on promoting CHO efflux;the alcohol precipitation has significant advantages in CHO conversion to bile acids.
陈星彤;杨金彪;周云越;薛爽;王乔;郭璇;张玉昆;牛雯颖
黑龙江中医药大学 基础医学院,黑龙江 哈尔滨 150040黑龙江中医药大学 基础医学院,黑龙江 哈尔滨 150040黑龙江中医药大学 基础医学院,黑龙江 哈尔滨 150040黑龙江中医药大学 基础医学院,黑龙江 哈尔滨 150040黑龙江中医药大学 基础医学院,黑龙江 哈尔滨 150040黑龙江中医药大学 基础医学院,黑龙江 哈尔滨 150040黑龙江中医药大学 基础医学院,黑龙江 哈尔滨 150040黑龙江中医药大学 基础医学院,黑龙江 哈尔滨 150040
高脂血症补阳还五汤ABCA1HMGCR胆固醇代谢
hyperlipidemiaBuyang Huanwu DecoctionABCA1HMGCRcholesterol metabolism
《中国中药杂志》 2026 (9)
2630-2641,12
国家自然科学基金项目(82274405)黑龙江省自然科学基金联合基金培育项目(PL2025H240)
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