针刺联合揿针对儿童单纯性肥胖胰岛素抵抗的影响OA

Objective:To evaluate the clinical efficacy of conventional acupuncture combined with press needle therapy in children with simple obesity(strong stomach and weak spleen pattern)and its impact on insulin resist-ance,and to preliminarily explore the potential mechanisms using bioinformatics analysis.Methods:A total of 98 children aged 6-16 years with simple obesity(strong stomach and weak spleen pattern)were randomly divided into a control group(receiving conventional acupuncture)and an observation group(receiving conventional acupuncture combined with press needle therapy).The treatment lasted for 12 weeks.Changes in body weight,body mass index(BMI),body fat percentage(BFP),waist circumference,fasting plasma glucose(FPG),fasting insulin(FINS),and homeostasis model assessment-insulin resistance(HOMA-IR)were compared before and after treatment be-tween the two groups.Additionally,transcriptomic data from the GEO database(GSE9624,GSE100599)were an-alyzed to investigate obesity-related abnormal signaling pathways and the potential regulatory mechanisms of acu-puncture.Results:A total of 11 cases dropped out.Ultimately,45 cases in the observation group and 42 cases in the control group completed this trial.Both groups showed significant reductions in body weight,BMI,BFP,and waist circumference(all P<0.05),with the observa-tion group demonstrating greater improvements in all indicators than the control group(all P<0.05).In terms of glucose metabolism,the decreases in FINS and HOMA-IR were significantly greater in the obser-vation group than in the control group(P<0.01).Bioinformatics analysis revealed that the PI3K-Akt and insulin signaling pathways were inhibited,while the Fo-xO and endocrine resistance pathways were activated in the obese state.Electroacupuncture intervention enriched pathways related to glucose and lipid metabolism and regulated hypothalamic central signaling.Conclusion:Conven-tional acupuncture combined with press needle therapy can safely and effectively improve body composition and insu-lin resistance in children with simple obesity(strong stomach and weak spleen pattern),with superior efficacy com-pared to conventional acupuncture alone.The mechanism of this combined therapy may involve modulation of the central nervous system and reversal of insulin signaling pathway abnormalities in peripheral adipose tissue.