基于SSR标记的荒漠锦鸡儿遗传多样性与环境适应性研究OA

[Objective]Based on simple sequence repeat(SSR)molecular markers,this study comprehen-sively analyzes the genetic diversity and population genetic structure of wild Caragana roborovskyi Kom.popu-lations.It further explores the association between genetic diversity and environmental factors,aiming to provide a scientific basis for the conservation and utilization of C.roborovskyi germplasm resources.[Method]Using SSR molecular marker technology,the genetic diversity of 10 wild populations of C.roborovskyi from four re-gions in China(Xinjiang,Gansu,Qinghai,and Ningxia)was analyzed.By calculating genetic diversity para-meters,and conducting population genetic differentiation analysis,genetic structure analysis,principal component analysis(PCA),cluster analysis,and Mantel test,the correlation between the population genetic structure and geographic distribution of C.roborovskyi was resolved.Additionally,correlation analysis in combination with en-vironmental factors was conducted to identify the key environmental factors affecting its genetic diversity.[Re-sult]The wild populations of C.roborovskyi exhibited high genetic diversity,with genetic variation primarily oc-curring within populations and frequent gene flow among populations(Nm=3.732 0).PCA and genetic struc-ture analysis showed that the 10 C.roborovskyi populations could be divided into two groups,while NJ cluste-ring analysis indicated no correlation between genetic distance and geographic distribution.The Mantel test con-firmed that there was no significant correlation between the genetic distance matrix and geographic distance ma-trix.Correlation analysis revealed that latitude,mean daily temperature range,and seasonal variation in precipita-tion were the main environmental factors affecting genetic diversity,suggesting that temperature fluctuations and precipitation patterns may be key drivers for shaping the spatial distribution of its genetic diversity.[Conclusion]This study firstly establishes a linkage framework between the genetic background of wild populations of C.ro-borovskyi and their environmental adaptation mechanisms.It is confirmed that species in arid habitats maintain genetic diversity through intra-population genetic variation and frequent gene flow,which provides a scientific ba-sis for formulating germplasm resource protection and management plans based on genetic information.