砂生槐提取物的杀虫活性及其生物碱含量分析OA

[Objective]The methanol,ethanol,ethyl acetate,acetone and petroleum ether were used to ex-tract different parts of Sophora moorcroftiana to analyze the insecticidal activity and the content of alkaloids in different solvent extracts of different parts,and to select the appropriate solvent for the extraction of insecticidal active ingredients in different parts of Sophora moorcroftiana.[Method]With Brevicoryne brassiace,Lipaphis erysimi,and the 3rd instar larvae of Plutella xylostella target insects,micro-dropping method was used to deter-mine the contact killing activity of extracts from different parts of Sophora moorcroftiana obtained by 5 different polar solvents(methanol,ethanol,ethyl acetate,acetone and petroleum ether).And the content of alkaloids(oxymatric,matricine,saponin,cytisine)in the methanol extract of different parts of Sophora moorcroftiana was analyzed by using high performance liquid chromography(HPLC).[Result]Results of insecticidal activity de-termination showed that the ethanol extract of Sophora moorcroftiana roots had the best contact killing activity against radish aphid,and the corrected mortality after 48 h of treatment reached 80.59%.The methanol extract of Sophora moorftiana seeds had the best contact killing activity against cabbage aphid,and the corrected morta-lity after 48 h of treatment reached 83.83%.The methanol extracts of Sophora moorcroftiana seeds and leaves had relatively high contact killing activity against the 3rd instar larvae of diamondback moth,and the mortality af-ter 48 h of treatment was 73.96%.HPLC analysis showed that oxidized saponin,matricine and saponin were de-tected in Sophora moorcroftiana seeds,leaves,stems and roots,and cytisine was not detected.The total con-tents of alkaloids in different parts of Soph moorcroftiana were ranked as:seeds>leaves>stems>roots,and the total content of alkaloids in Sophora moorcroftiana seeds was significantly higher than those in other parts.The contents of alkaloids in different parts of Sophora moorcroftiana were ranked as:oxymatricine>matricine>sa-ponin,and oxymricine accounted for more than 90%of the total alkaloids.The parts with higher total alkaloid content in the methanol extracts exhibited significantly stronger insecticidal activity than those with lower alka-loid content.The higher the total content of alkaloids,the better the insecticidal effect.[Conclusion]Manol and ethanol can be used as the preferred solvents for the extraction of insecticidal active ingredients of Sophora moor-croftiana.Different parts of Sophora moorftiana all contain oxymatricine,matricine and saponin,and the content of oxymatricine is significantly higher than that of matricine and saponin,accounting for more than 90%of the to-tal alkaloids.The total content of alkaloids in the seeds and leaves of Sophora moorcroftiana is higher than that in the stems and roots.