豌豆耐高温种质筛选及其响应高温胁迫的转录组分析OA

[Objective]This study aims to investigate the physiological responses and transcriptomic changes of peas under heat stress,providing a basis for breeding heat-tolerant pea varieties.[Method]A total of 29 pea germplasm resources were selected to measure physiological indicators under high-temperature stress,in-cluding relative chlorophyll content(SPAD value),antioxidant enzyme(SOD,POD,CAT)activities,malondi-aldehyde(MDA)content,proline(PRO)content,and protein content.Transcriptome sequencing was con-ducted on the main cultivated variety'Zhongwan 6'under high-temperature stress to identify differentially ex-pressed genes(DEGs).The expression patterns of key genes were further validated using RT-qPCR.[Result]High-temperature stress significantly impacted chlorophyll content,superoxide dismutase(SOD)activity,pro-line content,and protein content in peas.Through principal component analysis,membership function standard-ization,and cluster analysis,the 29 pea germplasm resources were categorized into three groups:heat-tolerant,intermediate,and sensitive.'Yunwan 59','Dianwan 1','Zhongwan 6',and'Nentai 8'were identified as heat-tolerant varieties.Transcriptome analysis revealed that high temperature stress induced significant expression changes in a large number of genes in pea leaves,with the number of DEGs increasing significantly over time.KEGG functional enrichment analysis highlighted that the MAPK signaling pathway,ribosome pathway,and glyoxylate and dicarboxylate metabolism pathway play crucial roles in the high temperature response of peas.GO functional enrichment analysis identified the following core genes involved in high temperature response:class I heat shock protein gene novel 200,ribulose bisphosphate carboxylase gene KIW84_036234,and glycosyl hydrolase gene KIW84_040407.[Conclusion]Peas exhibit heat tolerance through physiological and molecular mechanisms under heat stress.The identified heat-tolerant varieties and key genes can be used for the breeding of heat-tolerant pea varieties.