基于数据挖掘和网络药理学探讨含百合中成药治疗失眠的用药规律与作用机制OA

Objective To explore the medication rules and potential mechanism of action of Chinese patent medicines containing Baihe(Lilii Bulbus)in the treatment of insomnia based on data mining and network pharmacology.Methods Prescriptions of Chinese patent medicines containing Baihe(Lilii Bulbus)were collected and screened through the Yaozhi network.SPSS software was used to perform analyses of medication frequency,properties,flavors,and meridian tropism,association rules,and cluster analysis to identify the core drug combination.Databases including BATMAN-TCM and GeneCards were used to screen the active components of the core drug combinations,disease targets,and intersecting targets.STRING and DAVID were further employed to construct the protein-protein interaction(PPI)network and conduct pathway enrichment analysis.Results A total of 73 prescriptions of Chinese patent medicines containing Baihe(Lilii Bulbus)were included,among which 17 were for heart system diseases with insomnia as the primary symptom.The top five high-frequency drugs were Baihe(Lilii Bulbus),Fuling(Poria),Suanzaoren(Ziziphi Spinosae Semen),Yuanzhi(Polygalae Radix),and Danggui(Angelicae Sinensis Radix).Association rule analysis showed that the"Baihe(Lilii Bulbus)-Fuling(Poria)"pair had the highest support(64.706%),and it was identified as the core drug pair.Based on high-frequency drugs and cluster analysis,a core drug combination was constructed with"Baihe(Lilii Bulbus)-Fuling(Poria)"as the core,incorporating Suanzaoren(Ziziphi Spinosae Semen),Yuanzhi(Polygalae Radix),and Danggui(Angelicae Sinensis Radix).A total of 184 core active components were screened from the core drug combination,yielding 454 intersecting targets with insomnia.PPI network analysis screened out 81 core targets,including tumor protein 53(TP53)and protein kinase B1(AKT1).Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis mainly involved signaling pathways such as phosphatidylinositol 3-kinase-protein kinase B(PI3K-AKT)and mitogen-activated protein kinase(MAPK).Conclusion The treatment of insomnia with Chinese patent medicines containing Baihe(Lilii Bulbus)takes"Baihe(Lilii Bulbus)-Fuling(Poria)"as the core drug pair.The mechanism may involve multiple components such as curcumin and emodin acting on multiple targets including TP53 and AKT1,regulating multiple signaling pathways such as PI3K-AKT,and exerting intervention effects on insomnia through neuroendocrine regulation,anti-inflammation,and antioxidant pathways.