一测多评法同时测定烟草及烟草制品中8种多酚类化合物OA

In order to achieve rapid determination of polyphenols in tobacco and tobacco products for quality assessment,a quantitative multi-component analysis by single marker(QAMS)was proposed to simultaneously determine 8 polyphenols including neochlorogenic acid,scopolamine,chlorogenic acid,cryptochlorogenic acid,caffeic acid,scopoletin,rutin and kaempferol 3-O-rutinoside.The analysis was performed using high-performance liquid chromatography with diode array detection(HPLC-DAD)for a segmented detection program.Separation of the 8 polyphenols was achieved on a C18 column using a mobile phase consisting of methanol,water and acetic acid under a gradient elution mode.The flow rate was set at 1.0 mL/min,and the column temperature was maintained at 30℃.Using chlorogenic acid as the internal reference,the relative correction factors for the other 7 polyphenols were calculated.The mass fractions of the 8 polyphenols in tobacco and tobacco products were determined using both the external standard method and the QAMS method(calibrated via multi-point,slope,and single-point methods).The results showed that:1)Under the optimized conditions,the 8 polyphenols exhibited good linear relationships within their concentration ranges(R2＞0.999 9),and the limits of detection(LODs)and quantification(LOQs)were 0.01‒0.15 and 0.02-0.48 mg/L.The spiked recoveries ranged from 92.00%to 102.38%with relative standard deviations(RSDs)of 1.56%-4.10%.2)Using chlorogenic acid as the internal reference,the relative correction factors calculated by the slope correction method demonstrated good durability and reproducibility(RSDs<3.00%).Furthermore,the slope correction method achieved the highest consistency with the external standard method,yielding an average relative deviation no more than 3.21%.3)The established method is simple,accurate and suitable for the batch determination and quality evaluation of the 8 polyphenols in tobacco and tobacco products.