干细胞水凝胶治疗奶牛子宫内膜炎的作用效果分析OA

Based on the therapeutic effect of MSCs preparations in dairy cows with endometritis,this study was to explore the mechanism of the action of stem cell preparations in treating bovine endometritis.Twenty dairy cows were used and divided into a healthy group(n=5)and an endometritis group(endometritis without any treatment,n=5),a treatment group(endometritis with stem cell hydrogel preparation,n=5),and an adjuvant group(endometritis with stem cell free hydrogel preparation,n=5).Samples of the endometrial tissue were collected using a bovine endometrial sampler.Histological changes in the uterine epithelium of cows were observed through HE staining sections and transmission electron microscopy.Seven days after injection,the therapeutic effect was evaluated using bovine transrectal B-mode ultrasound.The protein contents of HMGB1,Caspase3,GSDME,IL-18,and IL-1 β in the cow tissues were measured by Western blot in four groups.The results of the HE staining and transmission electron microscopy showed that,in the cows with endometritis,the endometrial epithelial cells underwent pyroptosis.The results of the B-mode ultrasound showed that the stem cell hydrogel attached to the endometrium,and the echo phenomenon in the cow uterus reduced or disappeared after treatment.The results of Western blot showed that the protein concentrations of GSDME and Caspase-3 in the treatment group were significantly reduced,compared with the adjuvant group(P＜0.05).The protein con-centrations of GSDME and Caspase-3 in the treatment group showed no significant difference,compared with the healthy group(P＞0.05).The expression level of Caspase-3 protein in the treatment group was significantly lower than that in the inflammation group(P＜0.01).There was no significant difference in HMGB1 protein and inflammatory factors IL-18 and IL-1 β between the healthy group and the treat-ment group(P＞0.05).The protein expression levels of HMGB1,IL-18,and IL-1 β were significantly reduced in the treatment group,compared with the adjuvant control group and the inflammation group(P＜0.01).To sum up,the stem cell hydrogel preparation played a good therapeutic role by inhibiting the scorch death of uterine epithelial cells.MSCs preparation significantly reduced the expression of pro-inflammatory factors IL-18 and IL-1β by inhibiting pyroptosis of the uterine epithelial cells.In the context of prohibiting antibiotic abuse,the present results suggest a new direction for treating endometritis in intensive dairy farms.