根皮苷、三叶苷与根皮素的体外稳定性研究OA

Objective To investigate the in vitro stability of phlorizin,trilobatin,and phloretin.Methods High performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)was employed to evaluate the stability of phloridzin,trilobatin,and phloretin in buffer solutions of various pH values(1.4,4.0,6.86,and 7.86),simulated gastric and intestinal fluids,and rat gastric and intestinal con-tents.Results Phlorizin and trilobatin degraded rapidly in strong acidic conditions,with trilobatin degrading at a higher rate than phlorizin.In neutral environments,the degradation of both compounds slows down.Under weakly alkaline conditions,initial degradation was rapid,but subsequently levels off.In both artificial and rat gastrointestinal fluids,the degradation of phlorizin and trilobatin were accelerated and ac-companied by the generation of phloretin.However,phloretin itself degraded rapidly in gastric acid and intestinal fluid,possessing an ex-tremely short half-life.Conclusion Phlorizin and trilobatin possess poor stability in the gastrointestinal environment,where they are prone to hydrolysis into phloretin.Furthermore,since phloretin itself is highly unstable,this instability significantly compromises their bioavailability.