一株类NADC30/HP-PRRSV重组毒株HNxy23的分离鉴定与遗传演化分析OA
Isolation,identification,and genetic and evolutionary analysis of a recombinant NADC30-like/HP-PRRSV strain HNxy23
旨在了解河南地区猪繁殖与呼吸综合征病毒(PRRSV)的流行现状及变异情况.对在河南省信阳地区某疑似 PRRSV 阳性猪场获得的肺脏组织进行病毒检测和分离,结果在 MARC-145 细胞上成功分离到 1 株 PRRSV,命名为 HNxy23.对 HNxy23 序列分析显示,HNxy23 基因组全长15 049 bp,与美洲型经典毒株 VR-2332 同源性为 84.1%,与其他美洲型毒株 JXA1、NADC30、CHsk1401 和 15LN1 的同源性分别为 83.6%、90.2%、90.2%和91.0%.Nsp2 氨基酸序列比对结果显示,HNxy23 与NADC30 毒株相似存在131(111+1+19)个不连续的氨基酸位点缺失.GP5氨基酸序列比对结果显示,HNxy23 的抗原表位存在部分氨基酸突变.经重组分析表明,HNxy23 是谱系 1 的 NADC30 毒株与谱系 8 的高致病性类疫苗毒株 JXA1-P80 的重组毒株,重组区域为 5 392～6 969 bp.本研究结果丰富了河南地区 PRRSV 分子流行病学数据,也警示当前 PRRSV 的遗传变异正在加剧.
To investigate the epidemiological status and genetic variation of porcine reproductive and respiratory syndrome virus(PRRSV)in Henan Province,lung tissue samples obtained from a suspected PRRSV-positive pig farm in Xinyang were tested and subjected to virus isolation.PRRSV was successfully isolated on MARC-145 cells and designated as HNxy23.Subsequent sequence analysis of HNxy23 re-vealed the following facts:The full length of the HNxy23 genome was 15 049 bp.It possessed a homology of 84.1%with the classic American strain VR-2332,and had homologies of 83.6%,90.2%,90.2%,and 91.0%with JXA1,NADC30,CHsk1401,and 15LN1,respectively.The amino acid sequence alignment of Nsp2 showed that HNxy23,similar to the NADC30 strain,had a total of 131 discontinuous amino acid site deletions,including 111+1+19.GP5 amino acid sequence alignment revealed partial amino acid mutations in the antigenic epitopes of HNxy23.The recombination analysis confirmed HNxy23 to be a recombinant strain between lineage 1 NADC30 and lineage 8 highly pathogen-ic vaccine strain JXA1-P80,with a recombination region of 5 392 bp to 6 969 bp.
刘恒岩;毛思洋;王思宇;廖廷堯;王丽;顾伟伟;杨俊静
东北农业大学,黑龙江 哈尔滨 150030东北农业大学,黑龙江 哈尔滨 150030东北农业大学,黑龙江 哈尔滨 150030东北农业大学,黑龙江 哈尔滨 150030东北农业大学,黑龙江 哈尔滨 150030勃林格殷格翰动物保健(上海)有限公司,上海 200000东北农业大学,黑龙江 哈尔滨 150030
农业科技
猪繁殖与呼吸系统综合征病毒(PRRSV)河南省类NADC30毒株分离重组分析
porcine reproductive and respiratory syndrome virus(PRRSV)Henan ProvinceNADC30-likevirus isolationrecombina-tion analysis
《畜牧与兽医》 2026 (7)
88-98,11
大学生创新训练计划项目(SIPT项目)
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