饲用黄丝藻粉的致突变试验OA

The aim of this experiment was to evaluate the potential mutagenicity of Tribonema powder extracts as a feed additive through the Ames test,micronucleus test and sperm deformity test.In the Ames test,substance dose groups were set as 5.0,1.0,0.2,0.04,0.008 mg/dish.Positive control groups and negative control(sterile water)were set.Each treatment was set with two series of metabolic activation system(+S9)and non-metabolic activation system(-S9),in order to determine the number of returning mutant colonies of test bacteria.Each dose group had two replicates in each dish.In both the mouse bone marrow micronucleus test and the sperm abnormality test,the test substance was administered at three dose levels:5.0,2.5,and 1.25 g/kg.Concurrently,the positive control(cyclophosphamide)and the negative control(1%carboxymethyl cellulose solution)groups were established,and the sperm abnormality rate and types were counted.The results showed that,compared with the control group,the average number of revertant colonies in the Ames test was less than twice that in the negative control group,and they were in absent dose-response relationship.The micronucleus rate of bone marrow and sperm aberration rate showed no significant effect(P＞0.05)with the negative control group.The mutagenic test results were all negative.