脯氨酸羟化酶phd1的缺失增强斑马鱼的低氧耐受能力OA

This study aims to elucidate the role of proline hydroxylase phd1 in hypoxia tolerance in zebrafish.To this end,wild-type(phd1+/+)and phd1-deficient(phd1-/-)zebrafish were subjected to hypoxic conditions.Then,erythrocyte numbers were analyzed using o-dianisidine staining,and hypoxia-responsive gene expression was assessed via real-time quantitative PCR.Additionally,the survival of zebrafish larvae and adults was measured.The study initially vali-dated the conservation of the PHD1 protein in humans,mice,and zebrafish.Under hypoxic conditions,the number of erythrocytes in phd1-/-zebrafish increased significantly compared to phd1+/+zebrafish,and phd1-/-zebrafish larvae and adults exhibited enhanced tolerance to hypoxia.Furthermore,hypoxia-responsive genes were more strongly induced in phd1-/-zebrafish than in phd1+/+zebrafish.These findings suggest that knocking out phd1 enhances hypoxia tolerance in zebrafish,advancing our understanding of the biological function of this gene in fish and offering a molecular target for breeding hypoxia-tolerant fish strains.