貂源肺炎克雷伯菌毒力基因及耐药特征分析OA

Klebsiella pneumoniae can cause pneumonia in mink.It has seriously affected the development of mink breeding industry.The purpose of this study was to analyze the virulence and drug resistance of Klebsiella pneumoniae from mink,and to provide thearetical basis for investigating the pathogenicity and drug resistance of Klebsiella pneumoniae.In this study,the bacteria were isolated and identified by pure culture,gram staining and PCR method.The agar microdilution method was used to detected the drug resistance spectrum of the strain.The serotype was identified by PCR method.The whole genome DNA was extracted and sequenced.The composition and important com-ponents of the whole genome sequence were analyzed.The genotypes,drug resistance genes and virulence genes of the strain were analyzed by bioinformatics of whole genome.The results showed that the isolated strain was Klebsiella pneumoniae,which belonged to K2 serotype.The results of drug sensitivity test suggested that the strain was resistant to tetracycline,chloramphenicol,florfenicol,ampicillin,ce-ftazidime,cefoxitin,ciprofloxacin,enrofloxacin and kanamycin,showing multiple drug resistance.The assembled genome size was 5435.522 kb and contained one chromosome and one plasmid.The whole genome analysis showed that the strain belonged to ST86.The strain carried 7 virulence genes and 6 resistance genes.In this study,one Klebsiella pneumoniae strain was isolated.Serotype,drug sensi-tivity test and whole genome analysis showed that the strain was highly virulent and multi-drug resistant,which was related to the high pa-thogenicity of Klebsiella pneumoniae and was easy to cause infection in humans and animals.