基于MLST及菌索发育比较的蜜环菌鉴定方法OA

Armillaria mellea is a symbiotic fungus crucial for the growth and development of Gastrodia elata.Variations in strains of Ar-millaria mellea significantly impact the yield and quality of G.elata.However,their morphological similarity hinders the rapid selection of symbiotic fungal strains.Therefore,it is imperative to establish an identification method for superior strains of Armillaria mellea from the perspective of intellectual property protection.This study employed the Multilocus Sequence Typing(MLST)method based on the whole-genome sequence of a high-yield Armillaria mellea in the market.Based on this sequence,we designed 10 pairs of specific primers for PCR detection of 16 commercially available Armillaria strains,including the M3 strain.A method for identifying strains was developed based on the developmental characteristics of Armillaria mellea rhizomorphs,effectively identifying the M3 strain among 16 strains of Armillaria mellea.The results indicated that,using 10 pairs of specific primers for PCR detection of 16 strains of Armillaria mellea,distinct PCR de-tection results were obtained for 12 strains,allowing them to be distinguished from the M3 strain.The remaining 3 strains of Armillaria mel-lea exhibited the same PCR results as the M3 strain.Based on the morphological development of fungal hyphae,it was observed that the M3 strain exhibits multiple and elongated fungal hyphae branches,significantly more than the other three strains of Armillaria.Hence,this can serve as a distinctive feature for identifying the M3 strain.Through the aforementioned methods,the M3 strain of Armillaria can be identified and differentiated from the other 16 strains.Comparative identification of Armillaria based on multi-locus sequence typing and fungal hyphae development characteristics is a viable method,which holds great significance for selecting and protecting excellent Armillaria strains sym-biotic with G.elata.