绿色木霉菌对沙地花生产量和真菌群落多样性的影响OA
Effects of Trichoderma viride on the Yield of Peanuts and the Diversity of Fungal Communities in Sandy Soil
[目的]以辽宁彰武章古台风沙地花生(Arachis hypogaea)为对象,研究其对花育23、辽花608、青花6号3个花生品种土壤真菌群落多样性的影响.[方法]设置绿色木霉菌(Trichoderma viride)不同施用浓度梯度(0.75,1.5,3.0 g·m-²),测定不同浓度下花生产量,并进行真菌群落多样性分析.[结果]青花6号对菌剂响应最积极,高浓度处理产量显著提升,为最优适配品种;花育23各处理均稳定增产;辽花608仅在中浓度处理下略有提升.菌剂施用显著改变土壤真菌群落结构,且效应受菌剂浓度与花生品种的共同调节.与对照相比,低、中浓度菌剂处理提高土壤真菌的物种丰富度和多样性指数,高浓度处理在部分品种中表现为抑制作用.在门水平上,子囊菌门(Ascomycota)、被孢霉门(Mortierellomycota)和担子菌门(Basidiomycota)为优势类群.Venn分析结果显示,施用菌剂增加特有真菌OTUs数量,但随浓度升高而减少.LEfSe分析表明,低浓度处理组具差异标志物种最多,高浓度组明显减少.[结论]适宜浓度的绿色木霉菌有利于提高风沙地花生根际土壤真菌多样性并优化群落结构,但过高浓度可能降低群落复杂性.研究结果为风沙区花生地微生物调控及土壤生态修复提供了科学依据.
[Objective]This study investigated the effects of different application rates of Trichoderma viride(0.75,1.5,and 3.0 g·m⁻²)on soil fungal community diversity in three peanut cultivars(Huayu 23,Liaohua 608,and Qinghua 6)in the sandy land of Zhanggutai,Zhangwu County,Liaoning Province.[Method]Different application concentration gradients of Trichoderma viride(0.75,1.5,and 3.0 g·m⁻²)were established to determine peanut yield under different concentrations and to analyze fungal community diversity.[Results]The results showed that Qinghua 6 responded most positively to the microbial agent,exhibiting high fungal diversity and significantly increased yield under all concentrations,making it the optimal matching cultivar.Huayu 23 showed stable yield increase across all treatments,while Liaohua 608 only slightly increased under JUN1.5 treatment.The application of T.viride significantly altered the structure of soil fungal communities,and the effects were co-regulated by inoculum concentration and peanut cultivar.Compared with the control,low and medium concentrations increased the species richness and diversity indices of the soil fungal community,whereas the high concentration exhibited inhibitory effects in some cultivars.At the phylum level,Ascomycota,Mortierellomycota,and Basidiomycota were identified as the dominant fungal groups.Venn analysis indicated that microbial inoculation increased the number of unique fungal operational taxonomic units(OTUs),but this number decreased with increasing application rate.LEfSe analysis revealed that the low-concentration treatment enriched the highest number of differential indicator taxa,whereas the high-concentration treatment markedly reduced it.[Conclusion]Overall,an appropriate application rate of T.viride can enhance soil fungal diversity and optimize community structure in peanut fields of sandy regions,whereas excessive high concentrations may reduce community complexity.These findings provide a scientific basis for microbial regulation and soil ecological restoration in peanut-growing sandy land ecosystems.
吕刚;王一焯;王锋佰;张靖凡;孔涛
辽宁工程技术大学 环境科学与工程学院,辽宁 阜新 123000辽宁工程技术大学 环境科学与工程学院,辽宁 阜新 123000辽宁省沙地治理与利用研究所,辽宁 阜新 123000大连海洋大学 海洋科技与环境学院,辽宁 大连 116023辽宁工程技术大学 环境科学与工程学院,辽宁 阜新 123000
农业科技
绿色木霉菌花生真菌群落多样性风沙地
Trichoderma viridepeanutfungal diversitysandy land
《沈阳农业大学学报》 2026 (3)
78-88,11
辽宁省教育厅基本科研项目(自主选题服务地方项目)(LJ212410147029)
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