毛竹EIN3/EIL基因家族的鉴定及表达模式分析OA

[Objective]The EIN3/EIL(ethylene-insensitive 3/ethylene-insensitive 3-like)gene family plays critical roles in ethylene signal transduction,plant growth and development,and responses to biotic and abiotic stresses.However,the systematic characterization and functions of this gene family in moso bamboo(Phyllostachys edulis)remain poorly understood.This study aims to systematically investigate the molecular characteristics,evolutionary patterns,and stress response profiles of the EIN3/EIL gene family in moso bamboo through genome-wide identification,evolutionary analysis,and expression profiling,thereby providing a theoretical basis for functional validation and stress resistance breeding in this species.[Methods]An integrated bioinformatics workflow was applied to identify the EIN3/EIL gene family at the whole-genome level in moso bamboo.Comprehensive analyses of gene structure,evolutionary characteristics,and expression patterns were subsequently performed.[Results]A total of 14 EIN3/EIL genes were identified in the moso bamboo genome,unevenly distributed across 13 chromosomes.Phylogenetic analysis classified these genes into three distinct subfamilies,showing closer evolutionary relationships with homologs from rice(Oryza sativa)and maize(Zea mays)than from Arabidopsis(Arabidopsis thaliana).Interspecies collinearity analysis identified 19 homologous gene pairs between moso bamboo and rice,with significantly higher covariance compared to Arabidopsis.The gene structures contained 1 to 9 introns,and the distribution of conserved motifs exhibited subfamily-specific patterns.All encoded proteins contained EIN3,EIN3 superfamily,and PLN03109 domains.Promoter regions were enriched with hormone-responsive elements and light-responsive elements,with some also harboring low-temperature and salt-stress responsive elements.The Ka/Ks ratios for 14 homologous gene pairs were all less than 1,indicating that they were subjected to purifying selection pressure.Expression analysis revealed that PeEIL08,PeEIL09,and PeEIL12 were significantly upregulated under salt and drought stress conditions.Notably,PeEIL09 maintained high expression across multiple treatments,suggesting a broad-spectrum stress-responsive role.[Conclusion]The EIN3/EIL gene family in moso bamboo is evolutionarily conserved yet functionally differentiated.Its members are likely regulated by promoter cis-elements and play important roles in abiotic stress responses.Core genes such as PeEIL08,PeEIL09,and PeEIL12 represent potential molecular targets for enhancing stress resistance in moso bamboo breeding.This study provides important insights for further functional characterization of this gene family and for understanding the regulatory mechanisms of the ethylene signaling pathway in stress adaptation of moso bamboo.