贝莱斯芽孢杆菌Apcs-1全基因组测序分析及对土壤微生物的影响OA

[Objective]In previoaus studies,our research group isolated a strain of Apcs-1 with significant antagonistic activity against potato common scab.[Method]To further elucidate its biocontrol mechanism,whole-genome sequencing and analysis of strain Apcs-1 were performed in this study,and the effects of this biocontrol bacterium on soil microbial communities were also determined.[Results]The results showed that the complete genome of strain Apcs-1 is 3 929 735 bp in length with a GC content of 46.50%,and harbors multiple functional genes related to biocontrol traits such as antibiosis,growth promotion and induced resistance.AntiSMASH analysis predicted abundant biosynthetic gene clusters encoding lipopeptides,polyketides,siderophores and other secondary metabolites,indicating a strong potential to produce diverse antimicrobial substances.Prediction using the VFDB database revealed that the strain carries defensive virulence factors,nonspecific virulence factors,aggressive virulence factors,and virulence-related regulatory genes.Further analysis of rhizosphere soil microbial communities after application of the strain fermentation broth demonstrated that B.velezensis Apcs-1 significantly increased the abundance and community evenness of beneficial rhizosphere.microorganisms(e.g.,phylum Actinobacteria,class Bacilli),and significantly enriched genes associated with key metabolic pathways including amino acid metabolism and carbohydrate metabolism in soil.[Conclusion]This study reveals the genetic basis and biocontrol functions of strain Apcs-1 at the genomic level and from the perspective of microbial community effects,providing a theoretical foundation for the development and application of this strain in the green control of potato common scab.