辽宁省甜椒内源RNA病毒的鉴定、基因组全长序列扩增及系统进化分析OA

[Objective]In this study,pepper samples suspected of viral infection were collected from Shenyang,Liaoning Province.Bell pepper endornavirus(BPEV)was detected in the samples using small-RNA deep sequencing,which was designated as BPEV-Liaoning(BPEV-LN).The identification,full-length genome amplification and phylogenetic analysis of the endogenous RNA virus were performed.[Method]Through reverse transcription-polymerase chain reaction(RT-PCR)method,the segmented sequences of BPEV-LN genome were amplified,ultimately obtaining a full-length sequence of 14 728 bp(GenBank:PP842643).[Result]The nucleotide sequence homology analysis revealed that BPEV-LN exhibited the highest homology(99.21%)with the isolate BPEV-XJ(MH182675.1)from Xinjiang.Phylogenetic tree analysis indicated that BPEV-LN and BPEV-XJ isolates were aggregated into one branch,indicating the closest genetic relationship between them.Furthermore,based on the RNA-dependent RNA polymerase(RdRp)sequence of BPEV,specific detection primers were designed and used to test 19 pepper samples suspected of viral infection collected from the same location.BPEV was detected in 17 of these samples.[Conclusion]This study represents the first identification of BPEV in Liaoning Province,obtains the full-length genomic sequence of BPEV-LN and establishes an RT-PCR detection method.These findings provide a theoretical basis for understanding the origin and control of BPEV,as well as technical support for its identification and detection.