基于16S rRNA高通量测序技术分析黑毛肉牛瘤胃微生物多样性和功能预测的研究OA

The experiment aimed to investigate the microbial diversity and function in the rumen of Black-haired beef cattle.Fifteen Black-haired beef cattle(bulls)with the same feeding environment and conditions,the same age,and an average body weight of 650 kg were selected.A manual vacuum pump was used to extract their rumen fluid,the microbial community structure of rumen fluid samples was analyzed by 16S rRNA high-throughput sequencing technology,and used PICRUSt2 for functional prediction.The results showed that a total of 706 776 optimized sequences and 444 900 effective sequences were obtained through the Illumina Miseq sequencing platform.The total number of operational taxonomic units(OTUs)was 3 428,the average sequence length was 418 bp,and the coverage was above 98.68%.Alpha diversity analysis indicated that the Simpson index,Shannon index,Chao1 index,and Ace index were 0.21,5.15,1 114.22,and 1 109.09,respectively.Taxonomic analysis revealed that the dominant phyla in the rumen microbiota of Black-haired beef cattle were Bacteroidota(49.41%)and Firmicutes(41.54%),the dominant order was Bacteroidales(49.36%),and the dominant genus was Prevotella(19.49%).Functional prediction analysis showed that,compared with the Kyoto Encyclopedia of Genes and Genomes(KEGG)database,the rumen microbiota of Black-haired beef cattle was mainly concentrated in the metabolism pathway at level one.Compared with the Clusters of Orthologous Groups(COG)database,the functional categories were mainly focused on translation,ribosomal structure and biogenesis,amino acid transport and metabolism,cell wall/membrane/envelope biogenesis,and carbohydrate transport and metabolism.The study shows that this result provides a reference for further analysis of the metabolic functions and mechanism of action of rumen microorganisms in black-haired beef cattle.