酸藤子活性物质蒽贝素对雌性大鼠中枢性性早熟调节作用OA

Objective To investigate the regulatory effect of embelin on central precocious puberty in female rats,and to provide experimental basis for the development and application of anthracene in the corresponding fields.Methods The crystal of embelin was extracted from the dried fruit powder of Embelia laeta(L.)Mez.The purity of embelin extracted from Embelia laeta(L.)Mez was analyzed by HPLC with area comparison method.Thirty-six female SD rats(5-days-old)were randomly divided into normal control group,central precocious puberty model group,leuprorelin group with high,medium and low dose groups of embelin.Except for the normal group,the rats in the other groups were induced by danazol to form CPP model in 5-day-old female rats.The rats in the leuprorelin group and different doses of embelin group were treated with corresponding drugs.The weight gain of rats in each administration group was recorded.The regulation of serum FSH and LH were detected by ELISA.The morphological changes of uterus were observed by pathological HE staining.The expression of GnRH protein in hypothalamus was observed by immunohistochemical staining.The expression of PKC and FSH-β protein in pituitary tissues of rats was detected by Western blotting.Results Leuprorelin,high and medium doses of embelin could inhibit the weight gain of precocious puberty rats(P＜0.05 or P＜0.01).Low dose of embelin could inhibit the weight gain of precocious puberty rats until 30 days of age(P＜0.05).The levels of serum FSH and LH in the leuprolide group,the high-dose and medium-dose embelin groups were significantly lower than those in the precocious puberty model group(P＜0.05 or P＜0.01).Low-dose of embelin could only inhibit the serum FSH level of precocious puberty offspring rats(P＜0.05).Compared with the precocious puberty model group,the ovarian volume of the mice in the leuprorelin,high and medium dose groups was significantly reduced.The expression of GnRH protein in the hypothalamic arcuate nucleus of female rats in the leuprorelin group and the embelin intervention group was significantly down-regulated,which was significantly different from the precocious puberty model group.The expression of GnRH protein in the hypothalamic arcuate nucleus and PKC and FSH-β protein in pituitary tissues of precocious puberty rats were down-regulated by embelin.Conclusion Embelin can inhibit the changes of signs in central precocious puberty rats,regulate serum LH and FSH levels by down-regulating the expression of GnRH protein in hypothalamic arcuate nucleus,and restore the morphology and function of uterus and ovary in precocious puberty rats.