脉冲射频治疗腰段背根神经元的电生理耦合研究OA

To explore the physiological mechanism of the electrophysiological model of pulsed radiofrequency(PRF)and neuronal coupling in pain treatment,based on the Hodgkin-Huxley(HH)model,the electrophysiological models of healthy and pathological states(Na1.7 and Na1.8 channel conductance increased by 125%and 115%,respectively,and K channel conductance decreased by 85%)were constructed.At the same time,time-varying electric field stimulation combined with PRF(frequency 2 Hz,pulse width 20 ms,voltage 45 V and time 120 s)were coupled.The results showed that before treatment,the pathological model discharged 6 times every 500 ms without stimulation,and the discharge was persistent and irregular.When the stimulus was increased to 0.02 and 0.05 μA/cm2,discharges of 15 and 28 times per 500 ms occured,respectively,and the firing frequency increased with the increase of stimu-lus intensity.After treatment,there was no spontaneous discharge when no stimulation,and the resting membrane potential was-65 mV.However,when the external stimulation current density was 0.02 μA/cm2,discharges of 10 times per 500 ms were all close to the healthy model.This study can provide a key theoretical tool for the optimization of PRF treatment parameters and the study of pain treatment regimens.