北京地区羊肚菌白霉病病原及其生物学特性OA
Pathogen of white mold disease of Morchella sextelata in Beijing and its biological characteritics
为明确北京地区羊肚菌真菌性病害种类及其生物学特性,本研究通过组织分离法从感病的羊肚菌子实体中分离纯化病原菌,结合形态学与分子生物学手段进行鉴定,并通过研究不同温度、pH、碳源和氮源对病原菌菌丝生长速率、菌落形态及其产孢量的影响,系统分析其生长特性.结果表明:经形态学特征观察和 ITS、RPB2 和 LSU 基因序列分析,病原菌鉴定为长孢假单隔孢Pseudodiploospora longispora(相似度>99%),柯赫氏法则接种试验验证了病原菌的致病性和正确性;生物学特性研究表明,该病原菌最适生长温度为20℃,pH适应范围较广,pH 5-9均可生长,最适碳源为蔗糖,最适氮源为麦芽浸粉;在 20℃、pH 5 条件下,以葡萄糖为碳源、酵母浸粉为氮源时产孢量最高.本研究明确了北京地区种植的六妹羊肚菌主要的真菌病害病原是 P.longispora,并揭示其对羊肚菌的致病性及其生理生化特征,为病害精准防控提供理论依据.
The pathogen of fungal diseases endangering Morchella sextelata in Beijing was isolated and purified by using tissue isolation method.Morphological and molecular biology methods were used for identification of the pathogen.Suitable growing temperatures and pH,and the effects of carbon and nitrogen sources on the growth rate,colony morphology,and sporulation of fungal hyphae were systematically analyzed.Through morphological observation and analysis of ITS,RPB2,and LSU gene sequences,the pathogen was identified as Pseudodiploospora longispora(similarity>99%).The Koch's rule inoculation test verified the pathogenicity of the pathogen.It was confirmed that the optimal growth temperature of the fungus was 20℃,and the pH adaptation scope was wide,ranging from pH 5 to pH 9.The most suitable carbon source is sucrose,and the most suitable nitrogen source is malt extract powder.Under the conditions of 20℃and pH 5,the highest sporulation was attained by using glucose as the carbon source and yeast extract as the nitrogen source.This test provides theoretical reference for preventing and controlling the white mold disease of cultivated Morchella sextelata.
曲珈萱;张亚静;张殿朋;王守现;刘宇;律凤霞;卢彩鸽
牡丹江师范学院生命科学与技术学院,黑龙江 牡丹江 157011||北京市农林科学院植物保护研究所,北京 100097||北京市食用菌工程技术研究中心,北京 100097北京市农林科学院植物保护研究所,北京 100097||北京市食用菌工程技术研究中心,北京 100097北京市农林科学院植物保护研究所,北京 100097||北京市食用菌工程技术研究中心,北京 100097北京市农林科学院植物保护研究所,北京 100097||北京市食用菌工程技术研究中心,北京 100097北京市农林科学院植物保护研究所,北京 100097||北京市食用菌工程技术研究中心,北京 100097牡丹江师范学院生命科学与技术学院,黑龙江 牡丹江 157011北京市农林科学院植物保护研究所,北京 100097||北京市食用菌工程技术研究中心,北京 100097
羊肚菌真菌病害分离鉴定长孢假单隔孢生物学特性
Morchellafungal diseaseisolation and identificationPseudodipolospora longisporabiological characteristics
《菌物学报》 2026 (6)
165-177,13
现代农业产业技术体系北京市食用菌创新团队(BAIC03)国家食用菌产业技术体系(CARS20) This work was supported by the Beijing Edible Fungi Innovation Team of the Modern Agricultural Industrial Technology System(BAIC03)and the National Edible Fungi Industry Technology System(CARS20).
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