使君子化学成分的提取分离及其抗氧化活性OA

Objective The monomeric compounds in Quispualis indica were extracted and their structures were identified,and then their antioxidant effects were explored.Methods Pulverize Quisqualis indica,extract with ethanol under reflux,combine the filtrates,extract the filtrates with purified water,n-butanol,ethyl acetate and petroleum ether respectively,and separate the monomer compounds by high performance liquid chromatography C18 column.After detection,the chemical structure of the compound was identified by physicochemical properties and spectral analysis.Then,with BHT as the reference substance,the ability of the monomer compounds to scavenge DPPH·,ABTS·,O2-·,·OH,the chelating ability with metal ions(Fe2+),and the ability to reduce Fe3+were tested,and their protective effect on indirect DNA damage was studied.Results Four kinds of monomeric compounds were extracted from the Quispualis Indica,which were identified as betulinic acid,ethyl gallate,trigonelline hydrochloride and gallic acid.Among them,gallic acid and ethyl gallate scavenging DPPH·,ABTS·,O2-·,·OH these four free radicals,as well as with the metal ion(Fe2+)chelating ability and reducing Fe3+ability to show a good antioxidant capacity.Betulinic acid showed good antioxidant capacity in scavenging ABTS·,O2-·,·OH free radicals,chelating ability with metal ions(Fe2+)and reducing Fe3+ability.The chelation ability of trigonelline hydrochloride with metal ions(Fe2+)was the strongest,and its antioxidant capacity was weaker than that of the other three monomer compounds.The antioxidant capacity of the four monomer compounds was positively correlated with the concentration of the compound,and had a certain protective effect on the indirect damage of DNA by hydroxyl radicals.Conclusion The four monomeric compounds extracted from quispualis indica have a certain antioxidant capacity,among which the antioxidant capacity of gallic acid and ethyl gallate is better,the antioxidant capacity of betulinic acid and trigonelline hydrochloride is weaker,and the antioxidant capacity of the four monomeric compounds has a certain positive correlation with the compound concentration.