宁夏枸杞内生菌分离和鉴定OA

Lycium barbarum L.is an important medicinal and edible plant in China,rich in endophytic microbial resources.Taking the tissue culture seedlings of'Ningqi No.1'as the research object,through methods such as endophytic microbial isolation,cultivation purification,colony morphological observation,DNA extraction,PCR amplification,sequencing and comparison with the NCBI database,the endophytic microbial community of these seedlings was systematically isolated and identified.The differences in the endophytic microbial communities among different growth stages of the plants were also compared.Through isolation and identification,a total of 7 endophytic microorganisms were obtained.The composition characteristics of the microbial community showed that Alternaria Nees(relative abundance 38.2%)was the dominant fungus,and Sphingomonas(relative abundance 19.1%)was the dominant bacterium.It was also found that the abundance of Sphingomonas and Methylobacterium was closely related to the growth vigor of the host,and the detection rate of these microorganisms in the"dominant individual"(11.7%)was significantly higher than that in the"inferior individual"(4.3%).The research results indicated that the tissue culture seedlings of'Ningqi No.1'contained abundant endophytic microbial resources,and their community structure,especially the abundance of beneficial microorganisms,was closely related to the growth vitality of the host.This lays a theoretical foundation for in-depth research on the symbiotic relationship between endophytic microorganisms and Lycium barbarum and the development of new microbial agents.