星油藤种子多不饱和脂肪酸积累关键MYB转录因子的鉴定OA

Plukenetia volubilis is a high-quality woody oil crop.The MYB transcription factor family,one of the largest transcription factor families in plants,are widely involved in the regulation of plant growth and development,signal transduction and secondary metabolism.Based on the transcriptome data of P.volubilis seeds,bioinformatics methods were adopted in this study to systematically identify the members of the PvMYB family and analyze the correlation with polyunsaturated fatty acids.Family members were screened by homologous alignment of MYB conserved domains,and the physicochemical properties were analyzed using TBtools.A phylogenetic tree was constructed by the neighbor-joining method with MEGA 7.0 software,and the characteristics of protein conserved domains and motifs were analyzed combined with MEME and Batch CD-Search.Differential expression analysis and heat map visualization were per-formed via TBtools software,and SPSS 27 software was used to analyze the correlation between the expression levels of differential genes and the contents of linolenic acid and linoleic acid.The results showed that 57 members of the PvMYB family were identified and divided into 9 subgroups.PvMYB proteins were typical hydrophilic proteins,most of which were unstable proteins,and 10 conserved motifs were predicted in total.Correlation analysis demonstrated that PvAPL-3 and PvMYB1R1-4 were characterized as core negative regulatory genes,which showed extremely significant negative correlations with both fatty acids(absolute value of correlation coefficient≥0.98).In contrast,PvMYB44-6 functioned as a core positive regulatory gene,exhibiting extremely significant positive correlations with the two fatty acids,with correlation coefficients reaching 0.988 and 0.991,respectively.Furthermore,PvGAMYB-2 and PvGA-MYB-3 also displayed strong positive regulatory effects on these fatty acids.This study clarified the sequence charac-teristics and expression patterns of the PvMYB family and the potential association with polyunsaturated fatty acid ac-cumulation,which would provide a theoretical basis for the molecular mechanism of MYB regulating lipid synthesis in P.volubilis,and also supply key gene targets for the genetic improvement of lipid metabolism in this plant.