辣椒CaPC家族成员的全基因组鉴定及花药特异基因的挖掘OA
Genome-wide Identification of the Pepper CaPC Gene Family and Discovery of the Anther-specific Genes
蓝铜蛋白(phytocyanin,PC)是一类植物特有的 I 型铜结合蛋白,在植物生长发育过程中有着重要的作用.目前,PC 基因家族在辣椒中的全基因组鉴定和在花粉发育中的功能尚未见报道.本研究基于辣椒(Capsicum annuum L.)基因组和转录组数据,综合运用基因组学、生物信息学及分子生物学方法,对辣椒 PC 基因家族(CaPC)进行系统鉴定与功能初探.结果显示:辣椒基因组共包含 45 个 CaPC基因,不均匀分布于 11 条染色体,部分成簇分布.系统进化分析表明,CaPC 可分为 UCL、SCL 和 ENODL 三个亚家族,分别包含 6、8 和 31 个成员.基因结构和保守结构域分析表明,多数CaPC基因有 2 个外显子,所有 CaPC 成员均有 Cu_bind_like 保守结构域.全基因组共线性分析发现,辣椒基因组中存在 11 对由基因复制事件产生的 CaPC基因.近缘物种基因组共线性分析发现,辣椒、番茄和茄子的 PC共线性基因数目高度保守.启动子顺式作用元件分析表明,CaPC基因启动子区包含多个与光响应、激素应答、逆境响应及生长发育相关的调控元件.通过表达谱分析,共筛选出 4 个CaPC基因在花药中特异性表达,其中,CaENODL10.1在第 6～9 发育时期(Stage 6～9)的花药中特异性表达,此外,对CaENODL10.1进行 RT-PCR 分析发现,CaENODL10.1在质核互作雄性不育和细胞核雄性不育辣椒材料 Stage 7 发育时期的花药中均不表达,在与之相关的保持系中均表达,表明该基因与花粉的有无高度相关.综上所述,本研究对辣椒 CaPC 家族基因进行系统分析,研究结果为揭示PC基因在植物花粉发育中的作用提供新线索,也为辣椒雄性不育系的创制提供潜在分子靶点.
Phytocyanin(PC)is a class of plant-specific type I copper-binding proteins that play crucial roles in plant growth and development.To date,genome-wide identification of the PC gene family and its function in pollen devel-opment remain unreported in pepper.In this study,based on the genomic and transcriptomic data of pepper(Capsicum annuum L.),we conducted systematic identification and preliminary functional characterization of the PC gene family(CaPC)in pepper by integrating genomic,bioinformatic,and molecular biological approaches.The results revealed that the pepper genome contained a total of 45 CaPC genes,which were unevenly distributed across 11 chromosomes with partial clustering.Phylogenetic analysis indicated that CaPCs could be classified into three subfamilies,UCL,SCL and ENODL,comprising 6,8 and 31 members,respectively.Gene structure and conserved domain analyses showed that most CaPC genes contained two exons,and all CaPC members possessed the conserved Cu_bind-like domain.Whole-genome synteny analysis revealed that 11 pairs of CaPC genes were generated by segmental duplication in the pepper genome.Synteny analysis with closely related species indicated that the number of syntenic PC gene pairs was highly conserved among pepper,tomato,and eggplant.Promoter cis-acting element analysis showed that the promoter regions of CaPC genes harbored multiple regulatory elements associated with light responsiveness,hormone response,stress response,and growth and development.Expression profiling analysis identified four CaPC genes specifically expressed in anthers.Among them,CaENODL10.1 exhibited specific expression in anthers at developmental stages 6-9.Further RT-PCR analysis revealed that CaENODL10.1 was not expressed in anthers of either cytoplasmic-nuclear inte-raction male sterility(CMS)or genetic male sterility(GMS)pepper lines,suggesting that this gene may serve as a key factor in pollen development.In conclusion,this study conducted a systematic analysis of the CaPC family genes in pepper.The findings would provide novel insights into the role of PC genes in plant pollen development and offer po-tential molecular targets for the development of male sterile lines in pepper.
吴明华;董骥驰;赵晓圆;陈姝晓;程蛟文;胡芳;王俊;李琼;莫昊天;化青珠
仲恺农业工程学院园艺园林学院,广东 广州 510225仲恺农业工程学院园艺园林学院,广东 广州 510225华南农业大学园艺学院,广东 广州 510642华南农业大学园艺学院,广东 广州 510642华南农业大学园艺学院,广东 广州 510642韶关学院广东省粤北食药资源利用与保护重点实验室,广东 韶关 512005仲恺农业工程学院园艺园林学院,广东 广州 510225仲恺农业工程学院园艺园林学院,广东 广州 510225仲恺农业工程学院园艺园林学院,广东 广州 510225仲恺农业工程学院园艺园林学院,广东 广州 510225
农业科技
辣椒蓝铜蛋白花药特异基因雄性不育
pepperphytocyaninanther-specific genemale sterility
《热带作物学报》 2026 (6)
1398-1412,15
广州市科技计划项目(No.2025A04J4138)国家自然科学基金项目(No.32302562)广东省自然科学基金项目(No.2024A1515013247).
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