番茄酸性磷酸酶基因启动子的克隆及其表达分析OA

Acid phosphatase(ACP)is a key enzyme in plant phosphate metabolism,and elucidation of its gene expression regulation mechanism is of great theoretical value for improving plant phosphorus utilization efficiency.In this study,qPCR was used to detect the tissue expression pattern of SlACP(Gene ID:Solyc06g062380.3)in tomato(Solanum lycopersicum).The promoter of SlACP gene was cloned and fused to the GUS reporter gene in the p1300GN expression vector,and transgenic Arabidopsis thaliana was obtained via Agrobacterium tumefaciens-mediated transformation.The expression pattern of SlACP in transgenic plants were detected by GUS histochemical staining.Additionally,bioinformatics analysis was used to predict the cis-acting elements of the promoter.The qPCR results showed that SlACP was predominantly expressed in tomato roots.GUS histochemical staining showed that SlACP was highly expressed in Arabidopsis thaliana roots.Bioinformatics analysis revealed that promoter of SlACP contained multiple root-specific expression elements.The combination of GUS histochemical staining and qPCR results indicated that the promoter of the SlACP gene was a root-specific promoter.The findings above provide important theoretical basis and genetic resources for developing molecular breeding of phosphorus-efficient crops using this promoter.