低温胁迫下白菜型冬油菜BrSAMS2蛋白的鉴定与分析OA

Winter Brassica rapa is an important oilseed crop in China,with strong adaptability,excellent agronomic traits and high production efficiency.Low-temperature adversity ranks among the most critical abiotic constraints throughout its developmental stages.To understand the molecular mechanism of cold resistance of winter B.rapa,in this study,two-dimensional electrophoresis was employed to identify differentially expressed proteins in the shoot apical meristem of winter B.rapa'Longyou 7'exposed to low-temperature stress.Furthermore,bioinformatics approaches were utilized to perform functional annotation and pathway enrichment analysis on these differentially expressed proteins.The results showed that low-temperature stress induced 24 significantly differentially expressed proteins,among which 8 were up-regulated,10 were down-regulated,and 6 were induced expression proteins.Go annotation results revealed that the majority of differentially expressed proteins participated in the response to low-temperature stress,antioxidant defense,energy metabolism,and cell structure maintenance processes.Analysis based on the KEGG database uncovered the pivotal roles played by differentially expressed proteins in carbon metabolism,photosynthetic carbon fixation,as well as multiple amino acid biosynthetic pathways.S-adenosylmethionine synthase 2(SAMS2)gene was associated with low-temperature response and had the highest expression level at 12 h of low-temperature treatment.The encoded protein was a stable hydrophilic protein with a conserved domain,and its promoter region contained cis-acting elements such as low-temperature response.BrSAMS2 might participate in the low-temperature response by regulating methyl donors and ethylene synthesis.This study provides new candidate genes and protein targets for revealing the molecular mechanism of cold tolerance in winter rapeseed.