基于转录组解析斯里兰卡木薯花叶病毒侵染对木薯叶片水杨酸合成的调控作用OA

Cassava is an important tropical food and cash crop,but the production is severely threatened by Sri Lankan cassava mosaic virus(SLCMV).In this study,healthy and SLCMV-infected cassava cultivar NZ199 were used as mate-rials to systematically analyze the impact of SLCMV infection on gene transcriptional activity in cassava leaves.Tran-scriptome data analysis identified a total of 4728 differentially expressed genes.Compared with healthy leaves,4495 genes were up-regulated and 233 genes were down-regulated in infected leaves.Further analysis revealed that in in-fected leaves,the expression of key genes in the isochorismate synthase(ICS)pathway of salicylic acid biosynthesis was suppressed,while key genes in the phenylalanine ammonia-lyase(PAL)pathway were activated.Liquid chroma-tography-mass spectrometry(LC-MS)detection showed that,compared to healthy leaves,salicylic acid content in in-fected leaves increased by 51.98%,while the content of salicylic acid glucoside decreased by 10.95%.In conclusion,SLCMV infection inhibited the expression of genes such as ICS and PBS3 in the ICS pathway,while activating genes such as PAL,CNL and BEBT in the PAL pathway,ultimately leading to increased salicylic acid accumulation in cassava leaves.This study would provide a theoretical basis for understanding the molecular mechanisms of cassava's response to SLCMV and for breeding disease-resistant varieties.