焦曲霉SN56147次生代谢产物分离鉴定及其对灰葡萄孢的抑制作用OA
Isolation and identification of secondary metabolites from Aspergillus ustus SN56147 and their inhibitory effects on Botrytis cinerea
利用硅胶柱层析、凝胶柱层析和高效液相色谱等技术对焦曲霉(Aspergillus ustus)SN56147 次生代谢产物进行分离纯化.通过高分辨质谱(HRMS)、核磁共振氢谱(1H NMR)、碳谱(13C NMR)、二维谱(2D NMR)等波谱技术并结合相关文献数据对 10 个化合物的结构进行鉴定,其中化合物 1(Q-methylalbrassitriol)为全新的倍半萜类化合物,化合物 2～10 均为已知化合物.采用菌丝生长速率法测定10 个化合物对灰葡萄孢(Botrytis cinerea)的抑制活性,并分别通过伤口法和离体叶片法测定了活性化合物对草莓灰霉病的防治效果.结果显示:化合物3(6-ethyl-2,4-dihydroxy-3-methylbenzaldehyde)对灰葡萄孢具有较强的抑制作用,其EC50 值为27.14 μg/mL.离体叶片法测定结果表明,400 μg/mL 下化合物 3 对草莓的保护和治疗效果分别为 51.85％和59.26％,接近于阳性对照嘧霉胺,具备进一步研究的潜力.
Silica gel column chromatography,gel column chromatography and high-performance liquid chromatography were used to isolate and purify secondary metabolites from Aspergillus ustus SN56147.The structures of ten compounds were identified via high-resolution mass spectrometry(HRMS),1H NMR,13C NMR,2D NMR and literature data comparison.Compound 1(Q-methylalbrassitriol)was characterized as a new sesquiterpenoid,whereas compounds 2-10 were known substances.The mycelial growth rate method was employed to test the antifungal activity against Botrytis cinerea,and wound inoculation and detached leaf assays were conducted to assess the control efficacy of active compounds on strawberry gray mold.The results revealed that compound 3(6-ethyl-2,4-dihydroxy-3-methylbenzaldehyde)exerted strong inhibitory activity against B.cinerea with an EC50 value of 27.14 μg/mL.At 400 μg/mL,its protective and curative effects on strawberry were 51.85％and 59.26％respectively,close to those of the positive control pyrimethanil,suggesting its promising prospect for further investigation.
王梓源;于雅伦;邓芳勤;于志国
沈阳农业大学 植物保护学院,沈阳 110866沈阳农业大学 植物保护学院,沈阳 110866沈阳农业大学 植物保护学院,沈阳 110866沈阳农业大学 植物保护学院,沈阳 110866
化学化工
焦曲霉次生代谢产物分离鉴定灰葡萄孢抑菌活性草莓灰霉病
Aspergillus ustussecondary metabolitesisolation and identificationBotrytis cinereaantifungal activitystrawberry gray mold disease
《农药学学报》 2026 (3)
516-523,8
国家重点研发计划(No.2018YFD0800401). Supported by National Key R&D Program of China((No.2018YFD0800401).
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