产暗斑蛋与正常蛋蛋鸡血清生化指标、输卵管及肠道菌群的差异分析OA

[Objective]To explore the differences in serum biochemical indices,oviducts and intestinal microbiota between laying hens producing dark-spotted eggs and normal eggs.[Method]A total of 120 60-week-old Dawu Jinfeng laying hens were selected and divided into the normal egg group and the dark-spotted egg group.The differences of serum antioxidant indices,lipid metabolism indices,oviduct length and weight,and intestinal microbiota structure of laying hens were compared.[Result]Compared with the normal egg group,the activities of catalase(CAT),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),total antioxidant capacity(T-AOC)and malondialdehyde(MDA)content in serum of laying hens in the dark-spotted egg group increased to varying degrees,but the differences were not significant(P>0.05).Serum alanine aminotransferase(ALT)activity significantly increased(P<0.05),whereas there were no significant differences(P>0.05)in the contents of high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),triglyceride(TG),total cholesterol(TC),aspartate aminotransferase(AST)activity or other indices.Oviduct weight significantly decreased(P<0.05),while there was no significant difference(P>0.05)in oviduct length.There were no significant differences in the richness or phylum-level diversity of the intestinal microbiota(P>0.05);at the genus level,the relative abundance of unclassified Bacteroidetes significantly increased(P<0.05).[Conclusion]The occurrence of dark-spotted eggs is not related to the body's antioxidant capacity and lipid metabolism level,but the relationship with liver health,oviduct development status and intestinal microbiota composition deserves attention.