弥漫性中线胶质瘤中PP1α和p-ERK的表达及其与临床病理特征和预后的关系OA

Objective To investigate the relationship between the expression levels of PP1α,p-ERK and tumor invasion-related factors MMP2 and MMP9 in diffuse midline gliomas(DMG)with H3K27 alteration tissue and clinico-pathological features and prognosis.Methods 31 cases of DMG and 9 cases of normal brain tissue paraffin-embedded samples was collected.Immunohistochemistry was uesd to detect the expression of PP1α,p-ERK,MMP2,and MMP9.Relationship between PP1α,p-ERK,MMP2,and MMP9 expression and clinical pathological characteristics was analyzed.Results The expression levels of PP1α,p-ERK,MMP2 and MMP9 in 31 cases of DMG tissues were higher than those in the control group.High expression of PP1α was associated with age≤18 years(P=0.044)and loss expression of ATRX protein(P=0.020);high expression of p-ERK was associated with age≤18 years(P=0.018);high expression of PP1α was moderately correlated with the expression of p-ERK(C=0.380,P=0.035),and high expression of p-ERK was moderately correlated with the expression of MMP2(C=0.470,P=0.003).The Kaplan-Meier curve showed that patients with high expression of PP1α(P=0.002),high expression of p-ERK(P=0.028),age≤18 years(P=0.039),tumor located in non-thalamic midline region(P=0.027),histological grade of high level(P=0.019),no postoperative adjuvant therapy(P=0.002),and p53 expression≥10％(P<0.01)had a poorer prognosis.Cox multivariate analysis suggested that high expression of PP1α(HR:4.661;95％CI:1.219-17.825;P=0.024),no postoperative adjuvant therapy(HR:1.768;95％CI:0.456-6.857;P=0.041),and p53 expression≥10％(HR:4.233,95％CI:1.528-11.722;P=0.011)were independent risk factors affecting the prog-nosis of DMG patients.Conclusion PP1α and p-ERK is highly expressed in DMG tissues,and their expressions are correlated.Patients with high expression of PP1α have poor prognosis,and PP1α may play an important role in the ma-lignant progression of DMG tumors.