断血流的总黄酮提取工艺优化及质量评价OA

An orthogonal experiment was conducted to optimize the ethanol reflux extraction process of total flavonoids from Clinopodium polycephalum,and a stable method for determining the total fla-vonoid content was established.Based on this,quality evaluations were carried out on 6 batches of Cli-nopodium polycephalum medicinal materials from different sources.The results showed that the optimal extraction process was 70%ethanol volume fraction in 3 hours and 2 extractions;the RSD of precision tests,stability tests,repeatability tests,and sample recovery tests of the nitrous acid-sodium nitrate-sodi-um hydroxide colorimetric method were all less than 2%,indicating the accuracy and reliability of the method;the total flavonoid content of the 6 batches of Clinopodium polycephalum medicinal materials ranged from 0.067%to 0.154%,and the batch-to-batch coefficient of variation was as high as 32.72%.Even for medicinal material samples from the same origin,the total flavonoid content still showed signifi-cant differences,suggesting that the quality uniformity of Clinopodium polycephalum medicinal materi-als currently circulating in the online market is poor.