以美贺酒庄为对照的宁夏贺兰山东麓不同产区赤霞珠葡萄浆果代谢特征分析OA

To reveal the metabolic differences of Cabernet Sauvignon grapes from different sub-regions of the Helan Mountain's Eastern Foothill Wine Region of Ningxia and the regulatory mechanism of"terroir",ten-year-old Cabernet Sauvignon grapes from six representative wineries were selected as the research objects.The Meihe winery(M1)in the region was used as the control.Using liquid chromatography-mass spectrometry(LC-MS)non-targeted metabolomics technology,the differences in the composition of metabolites between the grape berries of these wineries and those of the Meihe winery were analyzed.Key different metabolites were screened and KEGG pathway annotation analysis was conducted.The results showed that a total of 9 categories of 3,546 metabolites were detected,among which organic oxidized compounds accounted for the highest proportion(11.48%),followed by fatty acids(10.77%),carboxylic acids and their derivatives(9.93%),and flavonoids(9.31%);The significant difference metabolites of the grape berries of the six wineries and the Meihe winery were mainly carboxylic acids and their derivatives,flavonoids,and organic oxidized compounds;KEGG pathway enrichment analysis showed that the most significantly different pathways between Renyiyuan winery(M2),Lilan winery(M3),and Meihe winery(M1)were oxidative phosphorylation,those between Yuquanying winery(M4)and Meihe winery(M1)were aminoacyl-tRNA biosynthesis,between Weijiani winery(M5)and Meihe winery(M1)were citric acid cycle(TCA cycle),and between Xige winery(M6),Luolanmage winery(M7)and Meihe winery(M1)were fructose and mannose metabolism.The study clarified the core different metabolite categories and key metabolic pathways of Cabernet Sauvignon grape berries from different sub-regions of the Helan Mountain's Eastern Foothill Wine Region of Ningxia,providing a theoretical basis for analyzing the"terroir"regulation mechanism of the wine grapes in this region and achieving precise quality regulation.