博落回内生真菌Penicillium turbatum的次级代谢产物OA

In order to research the secondary metabolitesof the endophytic fungus Penicillium turbatum from Bolehui,the fermentation was carried out using a potato glucose liquid medium.Comprehensive chromatographic methods such as HP20 macroporous resin,MCI GEL CHP20P macroporous resin chro-matography,and semi-preparative HPLC were employed to separate and purify the metabolites.On the basis of that,the antibacterial activities of the metabolites against five types of bacteria were further in-vestigated.The results showed that a total of 11 monomeric compounds were isolated,namely penicillic acid,dihydropenicillic acid,orcinol,orsellinic acid,1H-Indole-3-carbaldehyde,Methyl Indole-3-acetate,4,4'-dihydroxybenzophenone,4-(2-hydroxyethyl)phenol,3-methoxy-4-methyl-3,5-dihydro-1H-pyrrol-2-one,guanosine,and adenosine.The 11 compounds were obtained from Penicillium turbatum for the first time.Penicillic acid has certain inhibitory effect on Chromobacterium violaceum,Escherichia coli,Bacil-lus subtilis and Staphylococcus aureus,minimum inhibitory concentration values were 25,50,100 and 100 mg/L respectively.Dihydropenicillic acid has certain inhibitory effect on Staphylococcus aureus,and its minimum inhibitory concentration value is 100 mg/L.