基于肠道菌群探究补中益气汤治疗肌肉减少症的作用及机制OA

Objective To investigate the therapeutic effect of Buzhong Yiqi Decoction on sar-copenia and its regulatory mechanism on gut microbiota.Methods Thirty-two 7-month-old male C57BL/6 mice were randomly divided into blank group,model group,Buzhong Yiqi Decoction group,and nutritional support group,with eight mice in each group.The blank group received an e-qual volume of normal saline,while the other three groups were subcutaneously injected with dexam-ethasone(DXM,5 mg/kg,0.1 mL/10 g)to establish a sarcopenia model.During the treatment pe-riod,the blank and model groups received an equal volume of normal saline by gavage;the Buzhong Yiqi Decoction group received Buzhong Yiqi Decoction at 8.19 g/(kg·d)by gavage;and the nutri-tional support group received leucine at 0.520 g/(kg·d)and calcitriol at 0.065 μg/(kg·d)by gavage,for eight consecutive weeks.Behavioral and motor functions were observed.Gastrocnemius muscle cross-sectional area was measured using ultra-high-frequency small-animal ultrasound,and gut microbiota composition was analyzed by 16S rRNA sequencing.Results Compared with the blank group,the model group showed a reduction of 10.49 s in the fatigue rotarod endurance time and a decrease of 0.68 mm2 in gastrocnemius muscle cross-sectional area(P＜0.05).Compared with the model group,the Buzhong Yiqi Decoction group showed increases of 8.48 s and 0.59 mm2 in the aforementioned indices,respectively(P＜0.05);the nutritional support group showed increases of 8.45 s and 0.56 mm2,respectively(P＜0.05),although the degree of improvement was slightly less than that in the Buzhong Yiqi Decoction group.Gutmicrobiota analysis revealed that,compared with the model group,the Buzhong Yiqi Decoction group exhibited an increased ratio of Firmicutes to Bacteroidetes,reduced abundances of Lactobacillus and Lachnoclostridium,increased abundance of Dubosiella,and significant enrichment of Akkermansia;the nutritional support group only partially modulated the aforementioned microbiota.Conclusion Buzhong Yiqi Decoction exerts a significant therapeutic effect on sarcopenia model mice by increasing gastrocnemius muscle mass,prolonging fa-tigue endurance time,and improving motor function.The underlying mechanism of replenishing qi and strengthening the spleen and muscle may be associated with the modulation of gut microbiota.This study provides experimental evidence for elucidating the role of Buzhong Yiqi Decoction in a-meliorating sarcopenia from the perspective of gut microbiota.