睾丸相关高度保守的致癌长链非编码RNA抑制miR-383-5p促进前列腺癌细胞增殖能力的机制研究OA

Objective To investigate the mechanism of testis-associated highly conserved onco-genic long non-coding RNA(THOR)on promotion of the proliferation of prostate cancer(Pca)cells.Methods The expression of THOR in Pca tissue samples and prostate cancer cell lines was detected by qRT-PCR.The siRNA was used to interfere with THOR expression in PC3 and LNCaP cells,and CCK-8 and cell cloning assays were employed to assess cell proliferation capacity.The expression of miR-383-5p in Pca tissue samples and Pca cell lines was detected by qRT-PCR.Gene chips and bioin-formatics analysis were applied to analyze the targeting relationship between THOR and miR-383-5p,which was validated by a dual-luciferase reporter assay.After treating PC3 and LNCaP cells with miR-383-5p mimics and inhibitors,respectively,the Western blot was used to detect the targeting effect of miR-383-5p on insulin-like growth factor 2 mRNA-binding protein 1(IGF2BP1).The combined effects of THOR knockdown and miR-383-5p inhibitors on Pca cells were investigated,and the expression of IGF2BP1 was analyzed by Western blot.Results The expression of THOR was significantly upregulated in Pca tissues compared with adjacent normal tissues(P＜0.01),while the expression of miR-383-5p was significantly downregulated(P＜0.01).Downregulation of THOR expression significantly inhibited the proliferation of Pca cells.The miR-383-5p was downreg-ulated in Pca tissues and Pca cells.Among 65 Pca samples,the expression level of miR-383-5p was negatively correlated with that of THOR(r=-0.792,P＜0.001).After downregulating THOR expression in PC3 and LNCaP cells,the expression level of miR-383-5p significantly increased.Gene chip detection results indicated that miR-383-5p expression was significantly increased in PC3 cells transfected with si-THOR.Bioinformatics analysis revealed that miR-383-5p could interact with THOR through complementary sequences.The luciferase reporter assay results showed that miR-383-5p mimics significantly reduced the luciferase activity of the wild-type THOR reporter plas-mid(P＜0.05),showing no significant effect on the mutant type.THOR regulated the expression of IGF2BP1 by targeting miR-383-5p,thereby affecting the proliferation of Pca.Conclusion THOR inhibits miR-383-5p through a sponge effect,promoting the progression of Pca.