噬菌体vB_Pas_JXAU_S1与抗生素协同对生物被膜抑制作用研究OA

[Objective]Pseudomonas aeruginosa(P.aeruginosa)is a common multidrug-resistant opportunistic pathogen that poses a significant threat to public health and human well-being.Consequently,developing novel fungicides targeting multidrug-resistant bacteria has become an urgent need for controlling pathogenic contamination.[Method]In this study,P.aeruginosa was used as the host bacterium to isolate lytic phages from sewage,characterized their biological properties,analyzed their complete genomes,and determined their combined effects with antibiotics on inhibiting and eradicating biofilms.[Result]A lytic phage vB_PaS_JXAU_S1 of multidrug-resistant P.aeruginosa was successfully isolated.It belongs to the Siphoviridae family.Its optimal multiplicity of infection was 0.01,the latent period was 20 min,and the burst size was 110 PFU/cell.The phage exhibited good thermal and pH stability.The whole genome size of vB_PaS_JXAU_S1 comprises 42 685 bp and encodes 58 open reading frames.vB_PaS_JXAU_S1 showed synergistic effects(FICI≤0.5)when combined with ciprofloxacin,imipenem,and meropenem.Specifically,when combined with meropenem(1/8 MIC),the inhibition rate of biofilm formation within 24 h was 87.37%,and the eradication rate of mature biofilms(72 h)is 63.15%.[Conclusion]This study successfully isolated and identified a lytic P.aeruginosa phage,designated as vB_PaS_JXAU_S1.Its combination with antibiotics exhibits synergistic effects against P.aeruginosa biofilms,providing new insights and methodologies for developing phage-based biocontrol agents to combat contamination by multidrug-resistant pathogens.