湖南稷子田菁间混作对宁夏盐碱地土壤微生物群落多样性的影响OA
Effects of Echinochloa frumentacea-Sesbania cannabina intercropping and mixed cropping on soil microbial community diversity in saline-alkali land in Ningxia
[目的]盐碱化是全球面临的重大土地问题,探究湖南稷子与田菁间作和混作对盐碱地土壤微生物群落及理化性质的影响,为盐碱地生物改良提供理论依据.[方法]以湖南稷子(Echinochloa frumentacea(Roxb.)Link)单作(E)、田菁(Sesbania cannabina(Retz.)Pers.)单作(S)为对照,设置湖南稷子与田菁间作(E‖S)和混作(E×S)种植方式,通过测定土壤理化性质及采用Illumina PE250高通量测序,分析湖南稷子田菁间混作对土壤理化性质和根际微生物群落多样性的影响.[结果]4种种植方式均能显著降低土壤全盐含量和部分pH(P<0.05),但对土壤养分的影响差异不显著(P>0.05).微生物多样性分析显示,混作处理的细菌Faith's PD指数显著高于田菁单作(P<0.05),而间作和混作的真菌Pielou_e和Chao 1指数显著低于湖南稷子单作(P<0.05).门水平群落组成分析表明,间作和混作显著提高了变形菌门(增加2.13%～4.04%)和子囊菌门(提高1.10%～4.27%)的相对丰度(P<0.05),同时显著降低了担子菌门的丰度(降低5.25%～5.92%)(P<0.05).相关性分析揭示,细菌α多样性与全盐含量呈显著负相关(P<0.05),而真菌α多样性与pH、全磷含量显著正相关(P<0.05).担子菌门与土壤pH呈显著正相关(P<0.05),与全盐含量呈显著负相关(P<0.05).[结论]湖南稷子单作的全盐含量最低,间作有效提升土壤养分.湖南稷子与田菁间作和混作是一种可行的盐碱地生物改良策略,与单作相比,对土壤pH和含盐量的差异不大,但提高速效磷和速效钾含量,并通过优化微生物群落改善土壤生态功能,为盐碱地可持续利用提供了理论支撑和技术参考.
[Objective]Salinization is a major land problem in the world.The effects of the mixed action of millet and sesbania on soil microbial community and physical and chemical properties were explored in Ningxia Hui Autonomous Region,so as to provide theoretical basis for biological improvement of saline-alkali land.[Method]With monocropping(e)of Echinochloa frumentacea and monocropping(s)of Sesbania cannabina as control,intercropping(E ‖ S)and mixed cropping(E×S)were set up in Hunan.[Result]All the four planting methods can significantly reduce soil pH and total salt content(P<0.05),but the effects on soil nutrients are not significant(P>0.05).Microbial diversity analysis showed that the Faith's PD index of bacteria in mixed cropping was significantly higher than that of sesbania monoculture(P<0.05),while the Pielou_e and Chao 1 indexes of fungi in intercropping and mixed cropping were significantly lower than that of millet monoculture in Hunan(P<0.05).The analysis of community composition at phylum level showed that intercropping and mixed cropping significantly increased the relative abundance of Proteobacteria(increased by 2.13%-4.04%)and Ascomycetes(increased by 1.10%-4.27%)(P<0.05),while significantly decreasing the abundance of Basidiomycetes(decreased by 5.25%-5.92%)(P<0.05).Correlation analysis revealed that α diversity of bacteria was negatively correlated with total salt content(P<0.05),while α diversity of fungi was positively correlated with pH and total phosphorus content(P<0.05).Basidiomycetes were positively correlated with soil pH(P<0.05)and negatively correlated with total salt content(P<0.05).[Conclusion]Single cropping of millet has the lowest total salt content,and intercropping can effectively improve soil nutrients.Mixed cropping between millet and sesbania is a feasible strategy for biological improvement of saline-alkali land,which can significantly reduce soil pH,salt content,increase available phosphorus and potassium content,and improve soil ecological function by optimizing microbial community,providing theoretical support and technical reference for sustainable utilization of saline-alkali land.
廖珍玮;马进燕;李磊;许兴
宁夏大学 林业与草业学院,宁夏 银川 750021宁夏大学 林业与草业学院,宁夏 银川 750021宁夏大学 林业与草业学院,宁夏 银川 750021宁夏大学 林业与草业学院,宁夏 银川 750021
农业科技
盐碱地间作混作土壤理化性质土壤微生物群落多样性
saline-alkali landintercroppingmixed sowingsoil physical and chemical propertiessoil microbial community structure
《江西农业大学学报》 2026 (3)
837-850,14
"十四五"国家重点研发计划项目(2021YFD1900600) Project supported by National Key Research and Development Program of China(2021YFD1900600)
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