传染性单核细胞增多症患儿急性期与恢复期外周血T、B、NK细胞亚群的比较OA

Objective To detect peripheral blood T,B and NK cell subsets in children with in-fectious mononucleosis(IM)during the acute and convalescent phases,as well as in healthy chil-dren,and to analyze their distribution characteristics and dynamic changes in different disease stages.Methods A total of 60 children with IM were enrolled as the study subjects,including 20 in the a-cute phase(acute group)and 40 in the convalescent phase(convalescent group).Additionally,40 healthy children undergoing routine physical examinations were selected as control group.Flow cytome-try was used to measure the percentages of peripheral blood T,B and NK lymphocyte subsets,including total T lymphocytes(TTL),helper T cells(Th),cytotoxic T cells(Tc),naive cells,central memory cells(CM),effector memory cells(EM),terminally differentiated effector memory cells re-expressing CD45 RA(TEMRA),total B lymphocytes(TBL),memory B cells,plasmablasts,transitional B cells,natural killer(NK)cells,γδ T cells,CD4/CD8 double-positive T cells(DPT),αβ+CD4/CD8 double-negative T cells(αβ+DNT)and the CD4/CD8.The T,B and NK lymphocyte subsets a-mong the three groups were compared and analyzed.Results The percentages of TTL,CD4 TEM-RA,Tc and CD8 CM in both the acute and convalescent groups were significantly higher than those in the control group(P＜0.05).The percentages of Th,CD8 naive cells,CD8 TEMRA and the CD4/CD8 in both the acute and convalescent groups were significantly lower than those in the con-trol group(P＜0.05).Compared with the acute group,the percentage of CD4 CM in the convales-cent and control groups was significantly lower,while the percentage of CD4 naive cells was signifi-cantly higher(P＜0.05).The percentage of CD4 EM in the acute group was significantly higher than that in the convalescent group,and the percentage of CD8 EM was significantly higher than that in the control group(P＜0.05).The percentage of TBL in both the acute and convalescent groups was significantly lower than that in the control group(P＜0.05).In the convalescent group,the percentage of plasmablasts was significantly lower,whereas the percentage of transitional B cells was significantly higher compared to the control group(P＜0.05).The percentages of NK cells and αβ+DNT in both the acute and convalescent groups were significantly lower than those in the con-trol group(P＜0.05),and the percentage of DPT in the convalescent group was also significantly lower than that in the control group(P＜0.05).Conclusion Peripheral blood T,B and NK cell subsets are significantly abnormal in children with IM during the acute phase but gradually stabilize during the convalescent phase.Dynamic monitoring of detailed lymphocyte subsets and related im-mune indicators can provide an objective immunological basis for disease assessment,evaluation of therapeutic efficacy,and prognostic analysis.