酶辅助-超声协同提取甘蔗多酚工艺条件优化OA

Using sugarcane rind as raw material,polyphenols were extracted by enzyme-assisted ultrasonic synergistic extraction(EASE).On the basis of single-factor experiments,the Box-Behnken response surface methodology was employed to optimize four key process parameters:cellulase dosage,ultrasonic power,extraction temperature,and ultrasonic time.Analysis of variance showed that the influence of the factors on polyphenol yield followed the order of ultrasonic power＞cellulase dosage＞extraction temperature＞ultrasonic time,and the established quadratic polynomial regression model exhibited a good fit(R2=0.9746).The optimal process conditions were cellulase dosage of 0.45％,ultrasonic power of 350 W,extraction temperature of 52 ℃,and ultrasonic time of 35 min.Under these conditions,the polyphenol yield reached 4.79 mg/g±0.05 mg/g.Compared with traditional heat reflux extraction and single ultrasound-assisted extraction,the synergistic extraction significantly shortened the extraction time,and increased the polyphenol yield by 28.5％and 12.3％,respectively;the DPPH radical scavenging activity was also improved.The complementary effects between enzymatic cell wall disruption and ultrasonic cavitation-enhanced mass transfer effectively promoted the release of sugarcane rind polyphenols.This study provides technical support for the high-value utilization of sugarcane processing by-products and the development of functional food ingredients.