鱼腥草对急性肺损伤大鼠肺组织的保护作用OA

Aim:To investigate the protective effects of Houttuynia cordata Thunb.(HcT)on lung tissue of rats with a-cute lung injury(ALI)and to explore the underlying mechanisms.Methods:Sixty SD rats were randomly divided into 6 groups:normal control group,model group,dexamethasone group(5 mg/kg),low-dose HcT group(7.2 g/kg),medium-dose HcT group(14.4 g/kg),and high-dose HcT group(28.8 g/kg),with 10 rats in each group.Rats were administered the cor-responding treatment by gavage once daily for 5 consecutive days.Except the normal control group,rats in the other 5 groups received an intraperitoneal injection of lipopolysaccharide(LPS)5 mg/kg to induce ALI.Six hours after LPS injection,phys-iological status was recorded,and the lung index was measured.Histopathological changes in lung tissue were observed by HE staining.The levels of TNF-α,IL-1β,IL-6,and IL-10 in serum,bronchoalveolar lavage fluid(BALF),and lung tissue were determined by ELISA method.Glutathione peroxidase(GSH-Px)activity and total antioxidant capacity(T-AOC)in lung tissue were also measured.The mRNA expression of NLRP3,TNFAIP3,NFKBIA,and NF-κBp65 in lung tissue were detected by RT-qPCR,and the protein expression of NLRP3,NF-κB,and NF-κBp65 were analyzed by Western blot.Re-sults:Compared with the model group,the lung index and inflammatory cell infiltration reduced,alleviated alveolar septal thickening and pulmonary edema alleviated,and the pathological injury score decreased in 3 HcT groups.In addition,the levels of IL-1β,IL-6,and TNF-α in serum,BALF,and lung tissue decreased,and the mRNA expression of NLRP3,TN-FAIP3,NFKBIA,and NF-κBp65 and the protein expression of NLRP3,NF-κB,and NF-κBp65 downregulated;GSH-Px ac-tivity,T-AOC,and IL-10 levels increased(all P＜0.05).These effects were most pronounced in the high-dose HcT group(all P＜0.05).Conclusion:HcT may alleviate LPS-induced ALI in rats by enhancing antioxidant capacity and suppressing inflammatory responses,possibly through regulation of the NLRP3/NF-κB signaling pathway.