HAMA增强GelMA的可打印性能应用于数字光处理3D打印骨支架的研究OA

Objective:To enhance the printable performance of GelMA through hyaluronic acid methacrylate(HAMA)and use it in the photocuring 3D printing mechanism to prepare high-precision biological scaffolds.Methods:Based on adding different concentrations of HAMA in GelMA,3D printing was performed by using hydrogel inks with different concentrations and ratios.The situations of each printing result were observed.Through mechanical testing and Young's modulus calculation of the printed scaffolds,the effect of HAMA on the mechanical properties of GelMA was studied.Through degradation experiments,the influ-ence of HAMA on the degradation of GelMA ink was investigated and its effect on structural stability was studied.CCK-8,live/dead staining,and cytoskeleton experiments were carried out on each group of scaffolds to test the biocompatibility of hydrogel ma-terials in each group.Results:By adjusting the ratio of components,the printability and high fidelity of the ink were optimized,and a hydrogel ink suitable for digital light processing 3D printing was prepared.HAMA increased the hardness of GelMA,im-proved the printability,and slowed down the degradation rate of the scaffold.It was proved that GelMA-HAMA hybrid hydrogel obtained better printability performance while having good biocompatibility.Conclusion:HAMA promotes the printability of Gel-MA hydrogel in terms of mechanical properties and structural stability,providing a new idea for the precise printing of complex bio-logical structures.