神户肠杆菌P3的全基因组分析OA

Soil salinization is becoming increasingly severe,posing a serious threat to the normal growth and development of crops.Howe ver,salt-alkali-tolerant growth-promoting rhizobacteria in saline-alkali soils can effectively alleviate the stress caused by saline-alkali environment on crops.Enterobacterium Kobei P3 is a phosphate-solubilizing and salt-alkaline-tolerant strain isolated from saline-alkali soil in Egypt.The whole genome of strain P3 was sequenced using PacBio RSII single-molecule real-time(SMRT)and Illumina sequencing platforms.After genome assembly,gene prediction,functional annotation,and analysis of salt-alkaline-tolerant genes and amino acid synthesis pathway analysis were performed.The results showed that the total sequence length of strain P3 was 4 737 517 bp,with a GC content of 55.07％.It encoded 4 348 proteins,83 tRNAs,and 25 rRNAs.BLAST against the GO,NR,COG,KEGG,and Pfam databases revealed the presence of phosphorus-solubilizing functional genes,including three sn-glycerol-3-phosphate ABC transporters,three phosphate ABC transporters,two phosphate regulators,and one inorganic pyrophosphatase.Regarding the salt-alkaline tolerance mechanism,an ion homeostasis regulatory network compolsed of key genes was identified,including four Na+/H+antiporters,six Na+-transporting NADH dehydrogenases,four MFS family Na+transporters,and one glycine betaine transporter.In addition,metabolic pathway analysis confirmedhte existence of a proline synthesis pathway,indicating that the strain could enhance crop stress resistance by synthesizing proline,laying the foundation for the subsequent development of microbial agents using this strain.