4-二乙氨基苯甲醛诱导肠道屏障稳态失调的机制研究OA

Objective:To investigate the effects of 4-diethylaminobenzaldehyde(DEAB)on the intestinal epithelial barrier in mice and to explore the underlying molecular mechanisms.Methods:A total of 12 adult male C57BL/6 mice were randomly divid-ed into a control group and a DEAB group.The DEAB group received intraperitoneal injections of 10mg∙kg-1∙bw-1 DEAB,and the control group received intraperitoneal injections of corn oil.After 7 days,H&E staining and PAS staining were performed to ob-serve histopathological changes in mice intestinal tissues.Immunofluorescence staining was used to measure the expression levels of Lgr5 and ZO-1 proteins in the intestinal epithelium.RT-PCR was employed to detect mRNA expression levels of IL-22,Reg3γ,Reg3β,and Lgr5 in intestinal epithelial cells.Flow cytometry was used to quantify CD11c+MHC-Ⅱ+cells and CD3+cells in the intestinal lamina propria lymphocytes.The bacterial load in mesenteric lymph nodes and spleen was assessed using plate counting.Results:Compared to the control group,DEAB-treated mice exhibited significantly shorter intestinal villi,increased in-flammatory cells,and reduced goblet cells(P＜0.05).The expression of Lgr5 and ZO-1 proteins in intestinal crypts was signifi-cantly decreased(P＜0.01).The mRNA expression levels of IL-22,Reg3γ,and Reg3β in intestinal epithelial cells were signifi-cantly elevated(P＜0.05),and the mRNA expression level of Lgr5 was decreased(P＜0.05).The numbers of CD11c+MHC-Ⅱ+cells and CD3+cells in the intestinal lamina propria lymphocytes were significantly increased(P＜0.05).The bacterial load in mes-enteric lymph nodes and spleen was significantly elevated(P＜0.01).Conclusion:Dysregulation of the retinoic acid metabolic pathway leads to abnormal numbers of intestinal immune cells,impaired intestinal barrier function,and increased risk of bacterial infection.The underlying mechanism may be associated with dysregulated Lgr5 protein expression and disruption of tight junctions.